|
|
Immunophenotypic Characterization of Nodal Follicular Helper T Cell Lymphoma, Angioimmunoblastic-type |
WANG Chun-yan1,LIU Song-ya1,ZHU Li1,YI Shu-juan1,KUANG Dong2,XIAO Ming1,MAO Xia1,*() |
1 Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Immunotherapy Research Center for Hematologic Diseases of Hubei Province, Wuhan 430030, China 2 Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
|
|
Abstract Objective: To investigate the immunophenotypic characteristics of nodal follicular helper T-cell lymphoma, angioimmunoblastic-type (nTFHL-A), and to assess the diagnostic value of flow cytometry in nTFHL-A. Methods: A total of 227 patients were included in our study. They were initially diagnosed with nTFHL-A in the Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China, from January 2010 to August 2023. Results: The lack and decreased expression of surface CD3 and the CD7 deficiency were the most common immunophenotypeic abnormalities of pan-T cell markers in nTFHL-A, and CD10 expression was the most specific for nTFHL-A. Almost all neoplastic T cells had bright PD-1 expression and CD45RO expression. Flow cytometry may present false negative results in patients with CD3-positive or slightly decreased expression and meantime with CD7-positive or partial expression (tissue 11.3%, bone marrow 10.3%). Bright PD-1 expression can improve nTFHL-A detection and also help to differentiate it from other peripheral T-cell lymphomas. Conclusions: Flow cytometry is an effective tool to screen, identify or differentiate nTFHL-A.
|
Received: 09 October 2023
Published: 16 January 2024
|
|
|
|
[1] |
Swerdlow S H, Campo E, Harris N L, et al. WHO classification of tumours of haematopoietic and lymphoid tissues (revised 4th edition). Lyon: IARC, 2017.
|
|
|
[2] |
郭艳敏, 刘雪霏, 焦莉娟, 等. 血管免疫母细胞性T细胞淋巴瘤组织学分级与预后分析. 中华病理学杂志, 2019, 48(10):784-790.
doi: 10.3760/cma.j.issn.0529-5807.2019.10.007
pmid: 31594043
|
|
|
[2] |
Guo Y M, Liu X F, Jiao L J, et al. Angioimmunoblastic T-cell lymphoma: histopathological grading and prognosis. Chinese Journal of Pathology, 2019, 48(10):784-790.
doi: 10.3760/cma.j.issn.0529-5807.2019.10.007
pmid: 31594043
|
|
|
[3] |
de Leval L, Rickman D S, Thielen C, et al. The gene expression profile of nodal peripheral T-cell lymphoma demonstrates a molecular link between angioimmunoblastic T-cell lymphoma (AITL) and follicular helper T (TFH) cells. Blood, 2007, 109(11): 4952-4963.
doi: 10.1182/blood-2006-10-055145
pmid: 17284527
|
|
|
[4] |
Iwai Y, Okazaki T, Nishimura H, et al. Microanatomical localization of PD-1 in human tonsils. Immunology Letters, 2002, 83(3): 215-220.
doi: 10.1016/s0165-2478(02)00088-3
pmid: 12095712
|
|
|
[5] |
Yabe M, Gao Q, Ozkaya N, et al. Bright PD-1 expression by flow cytometry is a powerful tool for diagnosis and monitoring of angioimmunoblastic T-cell lymphoma. Blood Cancer Journal, 2020, 10(3): 1-5.
doi: 10.1038/s41408-019-0268-7
|
|
|
[6] |
Basha B M, Bryant S C, Rech K L, et al. Application of a 5 marker panel to the routine diagnosis of peripheral T-cell lymphoma with T-follicular helper phenotype. American Journal of Surgical Pathology, 2019, 43(9): 1282-1290.
doi: 10.1097/PAS.0000000000001315
pmid: 31283630
|
|
|
[7] |
Krishnan C, Warnke R A, Arber D A, et al. PD-1 expression in T-cell lymphomas and reactive lymphoid entities: potential overlap in staining patterns between lymphoma and viral lymphadenitis. The American Journal of Surgical Pathology, 2010, 34(2): 178-189.
|
|
|
[8] |
Wood B L, Arroz M, Barnett D, et al. 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry Part B: Clinical Cytometry, 2007, 72B(S1): S14-S22.
|
|
|
[9] |
中国抗癌协会血液肿瘤专业委员会. 流式细胞学在非霍奇金淋巴瘤诊断中的应用专家共识. 中华病理学杂志, 2017, 46(4): 217-222.
|
|
|
[9] |
Hematology Oncology Committee of China Anti-Cancer Association. Expert consensus on the application of flow cytometry in the diagnosis of non-Hodgkin’s lymphoma. Chinese Journal of Pathology, 2017, 46(4): 217-222.
|
|
|
[10] |
Attygalle A, Al-Jehani R, Diss T C, et al. Neoplastic T cells in angioimmunoblastic T-cell lymphoma express CD10. Blood, 2002, 99(2): 627-633.
doi: 10.1182/blood.v99.2.627
pmid: 11781247
|
|
|
[11] |
Wang C Y, Zhu L, Liu S Y, et al. PD-1 combined with TRBC1 and pan-T cell antibodies for robustly monitoring angioimmunoblastic T-cell lymphoma. Frontiers in Medicine, 2022, 9: 962428.
doi: 10.3389/fmed.2022.962428
|
|
|
[12] |
Alaggio R, Amador C, Anagnostopoulos I, et al. The 5th edition of the World Health Organization classification of haematolymphoid tumours: lymphoid neoplasms. Leukemia, 2022, 36(7): 1720-1748.
doi: 10.1038/s41375-022-01620-2
pmid: 35732829
|
|
|
[13] |
Craig Fiona E, Foon Kenneth A. Flow cytometric immunophenotyping for hematologic neoplasms. Blood, 2008, 111(8): 3941-3967.
doi: 10.1182/blood-2007-11-120535
pmid: 18198345
|
|
|
[14] |
鲁华东, 谢建兰, 张丽娜, 等. 血管免疫母细胞性T细胞淋巴瘤模式Ⅰ型临床病理学特征. 中华病理学杂志, 2022, 51(9):856-860.
doi: 10.3760/cma.j.cn112151-20211222-00925
pmid: 36097902
|
|
|
[14] |
Lu H D, Xie J L, Zhang L N, et al. Clinicopathological features of angioimmunoblastic T-cell lymphoma pattern Ⅰ. Chinese Journal of Pathology, 2022, 51(9):856-860.
doi: 10.3760/cma.j.cn112151-20211222-00925
pmid: 36097902
|
|
|
[15] |
Wang C Y, Mao X, Liu S Y, et al. The early diagnostic dilemma in angioimmunoblastic T cell lymphoma with excessive plasma cells proliferation. Case Reports in Medicine, 2021, 2021: 1-8.
|
|
|
[16] |
高雪, 黄文勇, 李文生, 等. 血管免疫母细胞性T细胞淋巴瘤伴霍奇金样细胞的临床病理分析. 中华病理学杂志, 2015, 44(8): 553-558.
|
|
|
[16] |
Gao X, Huang W Y, Li W S, et al. Clinicopathologic analysis of angioimmunoblastic T-cell lymphoma with Hodgkin/Reed-Sternberg-like cells. Chinese Journal of Pathology, 2015, 44(8):553-558.
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|