|
|
Preliminary Study of Bone Marrow Tumor Necrosis Factor-alpha (TNF-α) Level and Its Prognostic Value in Adult B-cell Acute Lymphoblastic Leukemia |
WEN Zhi-hao,JIN Yi-ying,YANG Ying,ZHANG Xin,BAI Yang-juan,LIAO Hong-yan() |
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China |
|
|
Abstract Objective: This study intends to investigate the levels of bone marrow tumor necrosis factor-alpha (TNF-α) in adult acute B-lymphoblastic leukemia (B-ALL) patients and the role of TNF-α in the efficacy of chemotherapy and tumor recurrence. Methods: Peripheral blood and bone marrow plasma samples were prospectively collected from 107 patients with B-ALL (disease group), 25 healthy donors (healthy controls), and 77 patients with other types of hematologic neoplasms (disease controls). Plasma TNF-α concentration was determined by chemiluminescence immunoassay. Gal-9 expression in leukemia cells from remission and relapse patients was determined by flow cytometry and TNF-α was co-cultured in vitro with primary cells from the patients to detect the changes in the expression of Gal-9. Results: Bone marrow TNF-α levels were elevated in B-ALL patients and TNF-α levels correlate with whether or not they are in remission from the first chemotherapy treatment. Bone marrow TNF-α concentrations at the time of initial diagnosis were lower in the first-time chemotherapy complete remission patients compared to those in non-complete remission patients. The leukemia cell Gal-9 was significantly up-regulated in relapsed patients and TNF-α induced Gal-9 up-regulation in vitro. Conclusions: This study reveals that bone marrow and peripheral blood TNF-α levels are elevated in adult B-ALL patients and induce up-regulation of Gal-9 in leukemia cells to promote disease progression and tumor relapse.
|
Received: 16 October 2023
Published: 16 January 2024
|
|
|
|
[1] |
Terwilliger T, Abdul-Hay M. Acute lymphoblastic leukemia: a comprehensive review and 2017 update. Blood Cancer Journal, 2017, 7(6): e577.
doi: 10.1038/bcj.2017.53
|
|
|
[2] |
苏燕, 尹青松. 基于表面抗原表达的急性B淋巴细胞白血病治疗的研究进展. 中国实验血液学杂志, 2021, 29(2): 648-652.
|
|
|
[2] |
Su Y, Yin Q S. Research progress in the treatment of acute B lymphoblastic leukemia based on surface antigen expression. Chinese Journal of Experimental Hematology, 2021, 29(2):648-652.
|
|
|
[3] |
Paul S, Kantarjian H, Jabbour E J. Adult acute lymphoblastic leukemia. Mayo Clinic Proceedings, 2016, 91(11): 1645-1666.
|
|
|
[4] |
杨占甲. Th17及CD4+CD25+调节性T细胞在急性白血病外周血中的表达及预后意义. 中国现代医学杂志, 2015, 25(23):66-70.
|
|
|
[4] |
Yang Z J. Expressions of Th17 cells and CD4+ CD25+ T regulatory cells in peripheral blood of acute leukemia patients and their prognostic significance. China Journal of Modern Medicine, 2015, 25(23):66-70.
|
|
|
[5] |
Liu L, Chang Y J, Xu L P, et al. T cell exhaustion characterized by compromised MHC class I and II restricted cytotoxic activity associates with acute B lymphoblastic leukemia relapse after allogeneic hematopoietic stem cell transplantation. Clinical Immunology, 2018, 190: 32-40.
doi: S1521-6616(17)30333-9
pmid: 29477343
|
|
|
[6] |
Sethi G. TNF: a master switch for inflammation to cancer. Frontiers in Bioscience, 2008(13): 5094.
|
|
|
[7] |
Tsimberidou A M, Estey E, Wen S J, et al. The prognostic significance of cytokine levels in newly diagnosed acute myeloid leukemia and high-risk myelodysplastic syndromes. Cancer, 2008, 113(7): 1605-1613.
doi: 10.1002/cncr.23785
pmid: 18683214
|
|
|
[8] |
Cao A, Alluqmani N, Buhari F H M, et al. Galectin-9 binds IgM-BCR to regulate B cell signaling. Nature Communications, 2018, 9(1): 1-18.
doi: 10.1038/s41467-017-02088-w
|
|
|
[9] |
Giovannone N, Liang J, Antonopoulos A, et al. Galectin-9 suppresses B cell receptor signaling and is regulated by I-branching of N-glycans. Nature Communications, 2018, 9(1): 1-17.
doi: 10.1038/s41467-017-02088-w
|
|
|
[10] |
Lee M, Hamilton J A G, Talekar G R, et al. Obesity-induced galectin-9 is a therapeutic target in B-cell acute lymphoblastic leukemia. Nature Communications, 2022, 13(1): 1-16.
doi: 10.1038/s41467-021-27699-2
|
|
|
[11] |
Steelman A J, Smith R 3rd, Welsh C J, et al. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. The Journal of Biological Chemistry, 2013, 288(33): 23776-23787.
doi: 10.1074/jbc.M113.451658
|
|
|
[12] |
Haferlach T, Kern W, Schnittger S, et al. Modern diagnostics in acute leukemias. Critical Reviews in Oncology/Hematology, 2005, 56(2): 223-234.
doi: 10.1016/j.critrevonc.2004.04.008
|
|
|
[13] |
Swerdlow S H, Campo E, Pileri S A, et al. The 2016 revision of the World Health Organization classification of lymphoid neoplasms. Blood, 2016, 127(20): 2375-2390.
doi: 10.1182/blood-2016-01-643569
pmid: 26980727
|
|
|
[14] |
中国抗癌协会血液肿瘤专业委员会, 中华医学会血液学分会白血病淋巴瘤学组. 中国成人急性淋巴细胞白血病诊断与治疗指南(2021年版). 中华血液学杂志, 2021(9):705-716.
|
|
|
[14] |
Hematological Oncology Professional Committee of Chinese Anti-Cancer Association, Leukemia Lymphoma Group, Hematology Branch of Chinese Medical Association. Guidelines for the diagnosis and treatment of acute lymphoblastic leukemia in adults in China (2021 edition). Chinese Journal of Hematology, 2021(9):705-716.
|
|
|
[15] |
Gallipoli P, Pellicano F, Morrison H, et al. Autocrine TNF-α production supports CML stem and progenitor cell survival and enhances their proliferation. Blood, 2013, 122(19): 3335-3339.
doi: 10.1182/blood-2013-02-485607
pmid: 24041577
|
|
|
[16] |
宋晓梅, 李化, 舒莉萍. 急性淋巴细胞白血病患儿细胞因子水平与疾病状态的关系研究. 国际检验医学杂志, 2021, 42(15): 1816-1820.
|
|
|
[16] |
Song X M, Li H, Shu L P. Relationship between cytokines levels and disease status in children with acute lymphoblastic leukemia. International Journal of Laboratory Medicine, 2021, 42(15): 1816-1820.
|
|
|
[17] |
石沁, 欧阳红梅, 宋建新. 血液病患者骨髓微环境和外周血部分细胞因子水平的对比. 医学信息, 2019, 32(13): 81-83.
|
|
|
[17] |
Shi Q, Ouyang H M, Song J X. Comparison of bone marrow microenvironment and peripheral blood cytokine levels in patients with hematological diseases. Medical Information, 2019, 32(13): 81-83.
|
|
|
[18] |
Witkowski M T, Dolgalev I, Evensen N A, et al. Extensive remodeling of the immune microenvironment in B cell acute lymphoblastic leukemia. Cancer Cell, 2020, 37(6): 867-882.e12.
doi: S1535-6108(20)30214-2
pmid: 32470390
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|