<i>Botryococcus braunii</i>: From Carbon Dioxide Fixation to the Biosynthesis of Alkanes, Alkenes and Terpenes

doi:10.13523/j.cb.2309025

China Biotechnology

2024, Vol. 44

Issue (2/3): 164-175 DOI: 10.13523/j.cb.2309025

Botryococcus braunii: From Carbon Dioxide Fixation to the Biosynthesis of Alkanes, Alkenes and Terpenes

YU Bohan,GAO Baoyan,ZHANG Hu,ZHANG Chengwu^**(

)

Research Center of Hidrobiology, Jinan University, Guangzhou 510632, China

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Abstract

Highly efficient bioproduction of hydrocarbons is one of the effective means to solve the shortage of petroleum and other liquid fuels, while microalgae-based oils are a reliable choice for the production of sustainable biofuels. Botryococcus braunii is an irregular unicellular colony belonging to the chlorophyta, which has received widespread attention for accumulating the large amounts of hydrocarbons, with a maximum content of up to 75% of its dry weight. In recent years, continued in-depth research into the biological characteristics and growth physiology of B.braunii, has improved the feasibility of its large-scale cultivation and industrial production of hydrocarbons. In this review, the potential of B.braunii as a novel oleaginous microalga for hydrocarbon production was briefly described from the aspects of biological characteristics, hydrocarbon synthetic pathways and regulatory factors, omics studies, and large-scale cultivation techniques. This could provide a reference for exploring the large-scale industrial production of biofuels based on B.braunii, thus accelerating the development and exploitation of this microalgal resource.

Key words： Botryococcus braunii Alkanes Alkenes Terpenes Omics Large-scale cultivation

Received: 21 September 2023 Published: 03 April 2024

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Cite this article:

YU Bohan, GAO Baoyan, ZHANG Hu, ZHANG Chengwu. Botryococcus braunii: From Carbon Dioxide Fixation to the Biosynthesis of Alkanes, Alkenes and Terpenes. China Biotechnology, 2024, 44(2/3): 164-175.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2309025 OR https://manu60.magtech.com.cn/biotech/Y2024/V44/I2/3/164

Table 1 Hydrocarbons and other lipids in the four races of B.braunii

Fig.1 Three B.braunii strains of different races 807-1: Race A, obtained from SAG; SC-1: Race B and SC-2, Race L, isolated from freshwater environments in South China; The cells in the left column were in the logarithmic phase, while those in the right column were in the stationary phase; Bar, 5 μm

Fig.2 Fatty acids, alkane, alkene and terpenoid hydrocarbons synthesis pathway of B.braunii

Fig.3 Common microalgae cultivation systems


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