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Preparation and Application of Monoclonal Antibody against Etomidate |
CHEN Fei1,2,WU Li-xian2,**(),GUO Jin-chen2,LIANG Fei-min2 |
1 Guangzhou Accurate and Correct Test Company, Guangzhou 510663, China 2 Guangzhou Wondfo Biotech Co., Ltd,Guangzhou 510663,China |
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Abstract Objective: To prepare anti-ET antibody for rapid detection of ET using the colloidal gold method. Methods: After identified by mass spectrometry and ultraviolet spectrum, the synthetic ET antigen was used to immunize Balb/C mice. ET hybridoma cell lines with high specificity were obtained by hybridoma technique. After purification by Protein G column, the antibody with appropriate sensitivity was selected and applied to ET colloidal gold reagent. The sensitivity and specificity of the antibody were identified by drug cross interference experiment and clinical experiment. Results: The antibody produced by 10F9 cell line was suitable for ET colloidal gold immunochromatography reagent. The ET reagent was used to detect 12 kinds of e-liquid additives and 48 kinds of commonly used drugs without cross-reaction. The cutoff of ET reagent in urine and e-liquid were both 500 ng/mL, the sensitivity was 100%, and the specificity was 99.5%.Conclusions: The monoclonal antibody (mAb) against ET was successfully prepared and applied to the colloidal gold immunochromatographic reagent.
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Received: 31 July 2023
Published: 01 December 2023
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Corresponding Authors:
**Li-xian WU
E-mail: lillian7040@126.com
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