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| Research Progress of Novel Diagnostic Methods of African Swine Fever |
ZHANG Yuan-yuan1,JIN Jia-xin1,ZHAO Xu-yang1,ZHANG Shuai1,LIU Yue-yue2,LI Xiang-lin3,SUN Ai-jun1,LIN Shu-qian2,**( ),ZHUANG Guo-qing1,**() |
1 International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China
2 The Shandong Animal Health Products Research Center, Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan 250100, China
3 Animal Husbandry and Veterinary Station of Xiadingjia Town of Longkou City, Yantai 265719, China |
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Abstract African swine fever (ASF) is a high-mortality infectious disease in pigs caused by the African swine fever virus (ASFV). ASF spreads in more than 40 countries around the world, and the ASF epidemic occurred in China in 2018, which caused serious economic losses to the pig industry. The World Organization for Animal Health (WOAH) lists it as a notifiable animal disease, and China lists it as a Class A infectious disease. Due to the complex genome structure and immune escape mechanism of ASFV, the development of ASF vaccine is difficult. No safe and effective ASF vaccine is available until now, and epidemic prevention and control mainly relies on accurate detection and strict biosecurity measurements. Therefore, the establishment of rapid, accurate and efficient diagnostic methods is essential for ASF prevention and control. In order to promote the research and development of new diagnostic methods to improve detection efficiency and clinical application, the current research progresses of ASF diagnostic methods are reviewed, focusing on the selection of diagnostic targets, as well as the research and development status, and advantages and disadvantages of etiological and serological detection methods. For example, there is introduction of droplet digital PCR, loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins system) and other new etiological diagnostic technologies and new serological detection methods that use nanomaterials, screening of dominant epitopes, and multi-antigen epitopes to improve sensitivity and specificity. Overall, this review provides a reference to develop new diagnostic methods for ASF epidemic prevention and control.
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Received: 09 May 2023
Published: 02 November 2023
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Cite this article:
ZHANG Yuan-yuan, JIN Jia-xin, ZHAO Xu-yang, ZHANG Shuai, LIU Yue-yue, LI Xiang-lin, SUN Ai-jun, LIN Shu-qian, ZHUANG Guo-qing. Research Progress of Novel Diagnostic Methods of African Swine Fever. China Biotechnology, 2023, 43(10): 62-71.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2305012 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I10/62
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