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| Research Progress of Subcellular Compartmentalization Regulation of Microbial Synthesis of Terpenes |
WAN Ya1,MENG Dong1,LI Jin-ling1,LI Chun1,2,WANG Ying1,**( ) |
1 Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering of Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China
2 Department of Chemical Engineering, Tsinghua University, Beijing 100084, China |
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Abstract Plant-derived terpenoids exhibit pharmacological activities such as anti-inflammatory and antioxidant effects, and inhibition of tumor cell proliferation, making them widely used in medicine. In recent years, microbial cell factories have gained significant attention for the synthesis of terpenoids. Efficient terpenoid synthesis in microorganisms requires regulation and optimization of metabolic athways. Subcellular compartmentalization is a common regulatory strategy that plays an essential role in constructing microbial cell factories. Subcellular compartmentalization of metabolic pathways enhances the concentration of enzyme and substrate, inhibits the transfer of metabolic flux towards by-products, reduces accumulation of toxic intermediates, and enables efficient terpenoid synthesis. Although the research on the synthesis of terpenoids using compartmentalization has been carried out, there is currently limited information summarizing its application in microbial cell factory construction. Therefore, this review summarizes the physiological characteristics of various organelles and their applications in regulating terpenoid synthesis. It also discusses the development of regulatory strategies, existing problems, and prospects of subcellular compartmentalization, aiming to provide references for the efficient biosynthesis of terpenoids.
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Received: 06 April 2023
Published: 08 October 2023
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