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Application Progress of Genetically Encoded Biosensors in Microbial Cell Factory |
HONG Xia1,2,TIAN Kai-ren3,QIAO Jian-jun1,2,3,LI Yan-ni1,2,**() |
1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China 2 Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin 300072, China 3 Zhejiang Institute of Tianjin University (Shaoxing), Shaoxing s312300, China |
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Abstract The rapid development of synthetic biology has promoted the biosynthesis of various complex chemicals in microbial cell factories. However, there are still many problems such as low yield and low production efficiency. Genetically encoded biosensors can sense the fluctuation of intracellular and extracellular metabolite concentration and external environment, and produce measurable signal output or regulate gene expression level in metabolic pathway. Biosensors have aroused widespread attention among synthetic biologists because of their advantages of low cost, simple operation and reproducibility. At present, genetically encoded biosensors have become an important part of synthetic biology and metabolic engineering, and also a powerful tool for metabolic dynamic regulation and ideal phenotype evolution/screening in microbial cell factories. Therefore, the composition and operating principle of genetically encoded biosensors are summarized first, and then, the latest application research of genetically encoded biosensors in dynamic regulation and high-throughput screening of microbial metabolism is emphatically introduced. Finally, the main challenges faced in the design and construction of genetically encoded biosensors are explored, and the future development prospects are discussed.
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Received: 08 March 2023
Published: 08 October 2023
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