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Oligonucleotide-based Biosenser for Highly Sensitive Detection of Hg2+ in Aqueous Solution |
Li-ping MA,Yun-xia LI,Ying-ying NIE,Sheng-long MA,Bo ZHENG**() |
Institute of Sensor Technology, Gansu Academy of Sciences, Key Laboratory of Sensor and Sensing Technology of Gansu, Lanzhou 730099, China |
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Abstract Objective: Mercury is a highly toxic and widespread pollutant in the environment, which can cause harm to human body even in low concentration. Based on the T-Hg2+-T base mismatch principle, a new biosensor was constructed to detect the concentration of Hg2+ in water with high sensitivity and selectivity. Methods: A new Gelred/TRO biosensor was constructed by using thymine rich oligonucleotide (TRO) as the specific Hg2+ recognition probe, and Gelred as the fluorescence indicator probe. The sensor is used to detect Hg2+ in water. The influence of many factors on the detection system was studied. Results: Under the optimized detection conditions, the linear range of Hg2+ detection was 10 ~ 800 nmol/L, which was fitted as follows: y=0.005 96x+0.881 44 (r2=0.991 47). The detection limit was 0.14 nmol/L. The presence of ten interfering ions such as Fe2+, Co2+, Cu2+, K+, Mg2+, Ca2+, Ag+, Cl-, , and did not affect the detection of Hg2+. The average recovery of Hg2+ in tap water samples and the Yellow River were 98.08% and 95.1%, respectively. Conclusion: This method has the advantages of simple operation, high sensitivity, good specificity and low detection limit. It has a good application prospect in the rapid determination of Hg2+ in drinking water.
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Received: 05 December 2022
Published: 03 August 2023
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