Propagation and Preservation of Germplasm Resources in <i>Pinellia ternata</i> (Thunb.)Breit.

doi:10.13523/j.cb.2209032

China Biotechnology

2022, Vol. 42

Issue (11): 18-26 DOI: 10.13523/j.cb.2209032

Propagation and Preservation of Germplasm Resources in Pinellia ternata (Thunb.)Breit.

JIA Ming-liang¹,FANG He-fang^1,^**(

),ZHANG Ben-hou²,HU Yan-hua²,ZHOU An-pei¹,LI Tong-jian¹,JIN Hong-guang¹,HAN Xing-jie¹,WEN Feng¹

1 Jiu Jiang University, Jiujiang 332000, China
2 Dafeng Marine Industrial Institute, Nanjing Tech University, Yancheng 224100, China

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Abstract

Objective: To provide technical support for the conservation, development and utilization of Pinellia ternata (Thunb.)Breit. (P. ternata) germplasm resources by studying the propagation and preservation methods of P. ternata. Methods: Firstly, the propagation conditions were explored by screening sterilization conditions, callus induction and cluster buds induction. Secondly, the suitable conditions for germplasm preservation were explored by setting different basic media, sucrose concentration, temperature and light intensities. Finally, the methods of awakening dormant tubers and reinducing clumping buds were explored to provide a basis for the re-propagation of germplasm resources. Results: The petiole explants were sterilized with mercuric chloride for 12 minutes and inoculated into the medium supplemented with 2,4-D to induce loose callus. The explants were inoculated to MS+2.0 mg/L 6-BA+0.1 mg/L NAA+ 30 g/L sucrose + 6.0 g/L agar, pH 5.8 for cluster buds induction. MS and N6 medium was the appropriate medium for germplasm preservation. The appropriate sucrose concentration is 30 g/L, and either too low or too high sucrose concentration was not conducive to germplasm preservation. It is necessary to provide sufficient temperature and light during tuber induction. Low temperature and light avoidance conditions are not conducive to germplasm preservation. It can be stored in vitro for up to 1-2 years after the sprout tumble of cluster buds. The wake-up induction of dormant tubers can be induced by cutting tubers and then inoculating them. The germination of the petiole can be directly induced by cluster buds proliferation, while the tubers can be inoculated into MS+ 6-BA 1.0 mg/L+NAA 0.2 mg/L+ sucrose 30 g/L+ agar 6.5 g/L, pH 5.8 to induce cluster buds re-differentiation. The cluster buds can be propagated or preserved in vitro. Conclusion: Through the propagation of germplasm of P. ternata, the dormant cluster tubers can be obtained by screening appropriate conditions, which can be preserved in vitro for a long time, and then awakened and induced again, providing a technical basis for the conservation and utilization of its germplasm resources.

Key words： Pinellia ternata (Thunb.)Breit. Germplasm resources Tissue culture and rapid propagation Germplasm preservation

Received: 14 September 2022 Published: 07 December 2022

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	Articles by authors
	JIA Ming-liang
	FANG He-fang
	ZHANG Ben-hou
	HU Yan-hua
	ZHOU An-pei
	LI Tong-jian
	JIN Hong-guang
	HAN Xing-jie
	WEN Feng

Cite this article:

JIA Ming-liang, FANG He-fang, ZHANG Ben-hou, HU Yan-hua, ZHOU An-pei, LI Tong-jian, JIN Hong-guang, HAN Xing-jie, WEN Feng. Propagation and Preservation of Germplasm Resources in Pinellia ternata (Thunb.)Breit.. China Biotechnology, 2022, 42(11): 18-26.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2209032 OR https://manu60.magtech.com.cn/biotech/Y2022/V42/I11/18

Table 1 Screening results of the sterilization conditions

Table 2 Effect of different hormone combinations on the petiole callus induction

Table 3 Effects of different hormone combinations in media on cluster buds induction

Table 4 Effects of different basic media on callus induction

Table 5 Effects of proliferation and dormancy of cluster buds under different basic media

Table 6 Proliferation and dormancy of cluster buds at different sugar concentrations

Table 7 Proliferation and growth of cluster buds at different temperatures

Table 8 Proliferation and growth of cluster buds under different light exposure

Table 9 Effects of cutting or not on germination of dormant tubers

Table 10 Effects of different hormone combinations on induction of tissue block cluster buds


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