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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2022, Vol. 42 Issue (1/2): 37-45    DOI: 10.13523/j.cb.2110003
Orginal Article     
Research Progress of Clostridium butyricum and Metabolic Modification of Butyric Acid Production
FU Yun-fei1,2,WEI Qi-lin2,YUAN Ming-gui2,KANG Hua-hua2,TIAN Ya2,XIANG Rong2,3,**(),XU Zhi-hong2,3,**()
1 Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
2 Guangdong Provincial Key Laboratory of Animal Disease Prevention, Scientific Observing and Experimental Station of Veterinary Drugs and Diagnostic of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
3 Zhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Zhaoqing 526238, China
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Abstract  

Clostridium butyricum is an obligate anaerobic bacterium, which can be modified by multiple genetic manipulation methods such as multi-gene overexpression, homologous recombination, homologous recombination based on non-replicating plasmids and non-replicating plasmids synthesis direction. Butyric acid is one of the fermentation products of Clostridium butyricum. It has a wide range of uses. It is used as a feed additive to improve animal resistance and reduce the use of antibiotics.The yield of butyric acid in the fermentation of Clostridium butyricum is still low, which is not conducive to industrial production. It is necessary to optimize the way of producing butyric acid by Clostridium butyricum through metabolic engineering.This article reviews the research progress of Clostridium butyricum’s main metabolic pathways, genetic manipulation systems, and the optimization of butyric acid synthesis pathways. On this basis, the thoughts and ideas for further transformation of Clostridium butyricum were prospected.

Key words： Clostridium butyricum      Metabolic pathway      Butyric acid      Modification     
Received: 05 October 2021      Published: 03 March 2022
ZTFLH:  Q812  
Corresponding Authors: Rong XIANG,Zhi-hong XU     E-mail: 23753842@qq.com;13922162272@139.com
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Yun-fei FU
Qi-lin WEI
Ming-gui YUAN
Hua-hua KANG
Ya TIAN
Rong XIANG
Zhi-hong XU
Cite this article:

FU Yun-fei,WEI Qi-lin,YUAN Ming-gui,KANG Hua-hua,TIAN Ya,XIANG Rong,XU Zhi-hong. Research Progress of Clostridium butyricum and Metabolic Modification of Butyric Acid Production. China Biotechnology, 2022, 42(1/2): 37-45.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2110003     OR     https://manu60.magtech.com.cn/biotech/Y2022/V42/I1/2/37

Fig.1 The butyrate metabolic pathway of Clostridium tyrobutyricum
Fig.2 The catabolism of glucose and xylose in Clostridium tyrobutyricum
菌株名称 改造策略 改造基因 目的
C.tyrobutyricum ATCC 25755 敲除 磷酸转乙酰酶基因(pta)和乙酸激酶基因(ack) 增加丁酸合成方向的代谢流
过表达 6-磷酸果糖激酶基因(pfkA)和丙酮酸激酶基因(pykA)
替换 丁酸关键酶基因thl替换了乙酸关键酶基因pta
过表达 Class I 型热激蛋白基因groESL、htpG 增加丁酸耐受性
过表达 醛酮还原酶基因(akr),短链脱氢酶/还原酶基因(sdr) 提高糠醛耐受性
克隆表达 丙酮丁醇梭菌ATCC 824中的scrA、scrB和scrK 提高对蔗糖的利用
丙酮丁醇梭菌ATCC 824中的galKET 提高对半乳糖的利用
丁酸杆菌Clostridium tyrobutyricum ATCC25755、大肠杆菌Escherichia coli BL21(DE3)、噬热厌氧杆菌Escherichia coli BL21(DE3)及嗜热厌氧杆菌Thermoanaerobacterium aotearoense SCUT27中编码转醛醇酶的tal基因 提高对木糖的利用
C.tyrobutyricum ATCC 25755/ ket 过表达 galKET,半乳糖非磷酸化转运蛋白基因(galP) 提高对半乳糖的利用
Table 1 Clostridium difficile transformation strateg
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