Preparation of Porphyrin Metal-organic Framework with Autophagy Inhibitory Effect and Its Photodynamic Cancer Treatment

doi:10.13523/j.cb.2107031

China Biotechnology

2021, Vol. 41

Issue (11): 48-54 DOI: 10.13523/j.cb.2107031

Preparation of Porphyrin Metal-organic Framework with Autophagy Inhibitory Effect and Its Photodynamic Cancer Treatment

HAN Xue-yi¹,LI Yi-fan¹,LU Yue-da¹,XIONG Guo-liang²,YU Chang-yuan^1,^***(

)

1 College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
2 Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China

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Abstract

Photodynamic therapy (PDT) has the advantages of minimally invasive, controllable, low toxicity, and repeatable treatment, and has become an indispensable treatment method in clinical medicine. However, due to the self-protection mechanism of tumor cells, the efficacy of PDT is greatly reduced. This study uses PDT therapy while implementing pharmacological autophagy suppression strategies to cut off protective autophagy under severe oxidative damage due to photodynamic therapy. The porphyrin metal organic framework PCN-224 was synthesized by the oil bath heating method, and the autophagy inhibitor hydroxychloroquine sulfate (HCQ) was loaded on the PCN-224, and the scanning electron microscope (SEM), particle size test (DLS), UV visible spectral testing and other methods showed that the material was successfully synthesized, which enhanced the water solubility of the porphyrin photosensitizer, and significantly enhanced the toxicity to 4T1 mouse breast cancer cells after light, and it further improved the tumor killing ability after being loaded with HCQ. HCQ can be released in tumor cells to deacidify lysosomes and inhibit autophagy, cut off the protective autophagy under severe oxidative damage due to photodynamic therapy, and achieve the effect of killing tumors.

Key words： Metal organic framework materials Photodynamic therapy Autophagy inhibition

Received: 13 July 2021 Published: 01 December 2021

ZTFLH:

R73-34

Corresponding Authors: Chang-yuan YU E-mail: yucy@mail.buct.edu.cn

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	Xue-yi HAN
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	Yue-da LU
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	Chang-yuan YU

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HAN Xue-yi,LI Yi-fan,LU Yue-da,XIONG Guo-liang,YU Chang-yuan. Preparation of Porphyrin Metal-organic Framework with Autophagy Inhibitory Effect and Its Photodynamic Cancer Treatment. China Biotechnology, 2021, 41(11): 48-54.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2107031 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I11/48

Fig.1 They are SEM representations of PCN-224 at different resolutions (a)Sample a (b)Sample b

Fig.2 Mean particle size of PCN-224

Fig.3 The UV-Vis spectra of nanoparticles

Fig.4 The standard curve of HCQ

Table 1 Loading efficiency& encapsulated efficiency of HCQ in MOF materials

Fig.5 The release rate curve of HCQ

Fig.6 Cytotoxicity of drugs (a)Dark group (b)Light group

Fig.7 Confocal imaging of active oxygen detection after illumination in PCN-224 treatment group and PCN-224+HCQ treatment group (a)ROS picture of PCN-224 treatment group (b)ROS picture of PCN-224+HCQ treatment group

Fig.8 The fluorescence picture autophagic vesicles (a)PCN-224 treatment group (b)PCN-224+HCQ treatment group


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