Preparation and Drug Release Properties of Keratin-loaded Nanoparticles

doi:10.13523/j.cb.2104002

China Biotechnology

2021, Vol. 41

Issue (8): 8-16 DOI: 10.13523/j.cb.2104002

Preparation and Drug Release Properties of Keratin-loaded Nanoparticles

LI Jia-xin¹,ZHANG Zheng¹,LIU He¹,YANG Qing¹,LV Cheng-zhi^2,^**(

),YANG Jun^1,^**(

)

1 School of Bioengineering, Dalian University of Technology, Dalian 116024,China
2 Dalian Dermatosis Hospital,Dalian 116021,China

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Abstract

Objective: Keratin was used as the drug carrier material, and the hydrophilic drug rhodamine and hydrophobic drug curcumin were loaded to prepare the intelligent responsive drug delivery system, and the pH and redox responsiveness of drug release were studied. Methods: The exopolysaccharide of Lactobacillus plantarum was introduced into the reaction system of sodium selenite and ascorbic acid, and KNP was synthesized at room temperature. The size, morphology, structure and stability of KNP were studied by tungsten filament scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and drug release experiments in vitro. Results:KNP with uniform particle size of about 300 nm was successfully prepared, which can load hydrophilic and hydrophobic drugs. Keratin based nanoparticles exhibited pH and glutathione (GSH) dual responsive release characters. In addition, these drug-loaded particles showed potent prolongation duration, good stability, sustainable and controllable release than the original drug. Conclusion: KNP with high stability, good water dispersion and responsiveness can be prepared simply and safely, and the drug delivery system of keratin is an appropriate candidate drug carrier.

Key words： Keratin Nanoparticles Drug carrier pH-responsive Redox-responsive

Received: 30 April 2021 Published: 31 August 2021

ZTFLH:

Q816

Corresponding Authors: Cheng-zhi LV,Jun YANG E-mail: dlpfb126.com;junyang@dlut.edu.cn

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	Jia-xin LI
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	Cheng-zhi LV
	Jun YANG

Cite this article:

LI Jia-xin,ZHANG Zheng,LIU He,YANG Qing,LV Cheng-zhi,YANG Jun. Preparation and Drug Release Properties of Keratin-loaded Nanoparticles. China Biotechnology, 2021, 41(8): 8-16.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2104002 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I8/8

Fig.1 Particle size of KNP under single factor condition (a)The influence of the proportion of dehydrating agent on the particle size of KPN (b)Effect of concentration of crosslinking agent on particle size of KPN (c)Effect of crosslinking time on particle size of KPN

Fig.2 Scanning electron microscope morphology of KNP tungsten filament (a) The SEM image of the KNP magnified 5 K times (b) The SEM image of the KNP magnified 20 K times

Table 1 KNP loading efficiency and zeta potential

Fig.3 Infrared spectra of drug-loaded nanoparticles (a)Infrared spectra of KNP and curcumin loaded KNP (b)Infrared spectrogram of KNP and rhodamine B loaded KNP

Fig.4 Release of drug-loaded nanoparticles at pH 5 and pH 7 in vitro (25℃) (a)Rhodamine B nanoparticles were released at different pH values (b)Curcumin nanoparticles were released at different pH values

Fig.5 Redox responsive release of drug-loaded nanoparticles in vitro at room temperature(25℃) (a)Redox responsive release of rhodamine nanoparticles (b)Redox responsive release of curcumin nanoparticles

Fig.6 Uptake of Rhodamine B nanoparticles in yeast cells (a)Rhodamine B with yeast were mixed for 6 hours (b)Rhodamine B nanoparticles with yeast were mixed for 6 hours (c) Rhodamine B with yeast were mixed for 24 hours

Fig.7 Uptake of curcumin nanoparticles in yeast cells (a)Curcumin nanoparticles with yeast were mixed for 6 hours (b)Rhodamine B nanoparticles with yeast were mixed for 24 hours


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