Engineering P450 for Specific Oxidation of Steroids

doi:10.13523/j.cb.20181213

China Biotechnology

2018, Vol. 38

Issue (12): 99-112 DOI: 10.13523/j.cb.20181213

Orginal Article

Engineering P450 for Specific Oxidation of Steroids

CHANG Qing-na^1,^2,³,YAO Ming-dong^1,^2,^3,^**(

),WANG Ying^1,²,XIAO Wen-hai^1,²,YUAN Ying-jin^1,²

1 Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China
2 SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China

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Abstract

As the second largest class of drugs in the world, steroidal drugs have important pharmacological activity such as anti-inflammatory, anti-allergic and endocrine regulation. Effective and specific oxidizing steroid nucleus by P450 is the key step to obtain pharmacological activity. As known, electron transport efficiency and catalytic specificity are important factors limiting P450 application, resulting in the low yield of target products and serious accumulation of by-products. Here, recent advances in the methodological strategy of engineering P450 were critically reviewed to improve the catalytic efficiency and specificity of steroidal oxidation. And it was also prospected the outlook on how to design and optimize steroidal P450 in the future.

Key words： Steroids P450 monooxygenase Electron transport Catalytic specificity

Received: 17 August 2018 Published: 10 January 2019

ZTFLH:

Q819

Corresponding Authors: Ming-dong YAO E-mail: 1982@163.com

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Cite this article:

CHANG Qing-na,YAO Ming-dong,WANG Ying,XIAO Wen-hai,YUAN Ying-jin. Engineering P450 for Specific Oxidation of Steroids. China Biotechnology, 2018, 38(12): 99-112.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20181213 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I12/99

Fig.1 Typical steroidal oxidation and the corresponding P450 monooxygenase

Fig.2 Engineering strategy of P450 catalytic system

Fig.3 Strategies for improving the efficiency of P450 electron transport

Table 1 Examples of adjusting the electron transport efficiency of P450

Fig.4 Strategies for improving P450 catalytic specificity

Table 2 Examples of improving the catalytic specificity of P450


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