The Research Progress of CRISPR-dCas9 in Transcriptional Regulation

doi:10.13523/j.cb.20180713

China Biotechnology

2018, Vol. 38

Issue (7): 94-101 DOI: 10.13523/j.cb.20180713

The Research Progress of CRISPR-dCas9 in Transcriptional Regulation

Kai-ren TIAN^1,^2,³,Er-shu XUE^1,^2,³,Qian-qian SONG^1,^2,³,Jian-jun QIAO^1,^2,³,Yan-ni LI^1,^2,^**(

)

1 School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
2 Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China
3 Syn Bio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China

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Abstract

The CRISPR/Cas9 system is not only a revolutionary tool for gene editing but also regulates gene transcription in various prokaryotic and eukaryotic organisms. In recent years,the system of CRISPR-dCas9 derived from CRISPR/Cas9 has been used in many fields such as gene imaging, high-throughput screening, gene regulation, investigating essential gene function and epigenetic regulation.The recent advances of CRISPR-dCas9 for activating or inhibiting gene transcription, reducing off-targeting efficiency and combing the intrinsic relationship between sgRNA and transcriptional regulation, application in the life science and further upgrading were described.

Key words： CRISPR-dCas9 Transcriptional regulation Transcriptional activation Transcriptional inhibition

Received: 15 March 2018 Published: 13 August 2018

ZTFLH:

Q789

Corresponding Authors: Yan-ni LI E-mail: liyanni@tju.edu.cn

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Cite this article:

Kai-ren TIAN,Er-shu XUE,Qian-qian SONG,Jian-jun QIAO,Yan-ni LI. The Research Progress of CRISPR-dCas9 in Transcriptional Regulation. China Biotechnology, 2018, 38(7): 94-101.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180713 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I7/94

Fig.1 The mechanism of CRISPRa and different types of CRISPRa activation systems (a)The mechanism of CRISPRa activates gene transcription (b)The dCas9-VP64 system (c)The dCas9-VPR or (VP16)n system (d)The dCas9-SunTag system (e)The SAM system (f)The dCas9-p300core system

Fig.2 The mechanism of CRISPRi and different types of CRISPRi inhibition systems (a)The mechanism of CRISPRi inhibits gene transcription (b)The dCas9-KRAB system (c)The dCas9-LSD1 system


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