Establishment and Identification of Endogenous CD133 <sup>+</sup> Cell Tracer Mouse Model

doi:10.13523/j.cb.20180608

China Biotechnology

2018, Vol. 38

Issue (6): 58-62 DOI: 10.13523/j.cb.20180608

Establishment and Identification of Endogenous CD133 ⁺ Cell Tracer Mouse Model

Ming-ming HAN,Yu-ping LUO(

)

School of Life Science,Nanchang University,Nanchang 330031,China

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Abstract

Objective:To establish a mouse model that can trace the differentiation of CD133 positive neural stem cells.Methods:The CD133-Cre-ERT2 transgenic mice were mated with Rosa26-CAG-LSL-ZsGreen transgenic mice to produce CD133-CreER;CAG-ZsGreen double transgenic in C57B16 mice.Result:The immunohistochemistry and laser scanning confocal imaging analysis showed that Tamoxifen-Inducible Cre/loxP recombination, as depicted by green cells, existed in lateral ventricle SVZ, the ventricular zone of third ventricle and the fourth ventricle in adult mice, and the green fluorescence overlaps with CD133⁺ red fluorescence in these regions.Conclusion:CD133⁺ is the sign of the resting nerve stem cell in the ventricle membrane, so it can trace the cell differentiation lineages of neural stem cells by analyzing ZsGreen positive cells in CD133-CreER;CAG-ZsGreen mice. The mouse model for in vivo lineage tracing of endogenous CD133⁺ cell was established successfully, which will be helpful to explore the activation, proliferation, migration and differentiation of CD133⁺ neural stem cells in the brain.

Key words： CD133 Neural stem cells Transgenic mouse

Received: 24 December 2017 Published: 06 July 2018

ZTFLH:

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Corresponding Authors: Yu-ping LUO E-mail: luoyuping@163.com

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Ming-ming HAN,Yu-ping LUO. Establishment and Identification of Endogenous CD133 ⁺ Cell Tracer Mouse Model. China Biotechnology, 2018, 38(6): 58-62.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180608 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I6/58

Fig.1 PCR genotyping for transgenic mice (a)~(b) CD133-Cre-ERT2 transgenic mice (c) Rosa26-CAG-LSL-ZsGreen transgenic mice

Fig.2 Schematic diagram of transgenic mice for tracing CD133⁺ cell

Fig.3 The immunochemistry staining show the distribution of ZsGreen and CD133⁺ NSCs in the mouse brain(a) The immunochemistry staining for lateral ventricle (b) The immunochemistry staining for third ventricle (c) The immunochemistry staining for fourth ventricle (blue:DAPI,green:ZsGreen,red:CD133)


[1]	Mckay R D , McKay R . Stem cells in the central nervous system. Science, 1997,276(5309):66-71. doi: 10.1029/97JD02210 pmid: 9082987

[2]	Yun S, Reynolds R P, Masiulis I, Eisch A J . Re-evaluating the link between neuropsychiatric disorders and dysregulated adult neurogenesis. Nat Med, 2016,22(11):1239-1247. doi: 10.1038/nm.4218 pmid: 5791154

[3]	Walter C, Murphy B L, Pun R Y , et al. Pilocarpine-induced seizures cause selective time-dependent changes to adult-generated hippocampal dentate granule cells. J Neurosci, 2007,27(28):7541-7552. doi: 10.1523/JNEUROSCI.0431-07.2007 pmid: 17626215

[4]	Unger M S, Marschallinger J, Kaindl J , et al. Early changes in hippocampal neurogenesis in transgenic mouse models for alzheimer’s disease. Mol Neurobiol, 2016,53(8):5796-5806. doi: 10.1007/s12035-016-0018-9 pmid: 5012146

[5]	Ming G L, Song H . Adult neurogenesis in the mammalian brain: Significant answers and significant questions. Neuron, 2011,70(4):687-702. doi: 10.1016/j.neuron.2011.05.001 pmid: 3106107

[6]	Fuentealba L C, Obernier K, Alvarez-Buylla A . Adult neural stem cells bridge their niche. Cell Stem Cell, 2012,10(6):698-708. doi: 10.1016/j.stem.2012.05.012 pmid: 3726005

[7]	Gage F H . Mammalian neural stem cells. Science, 2000,287(5457):1433-1438. doi: 10.1126/science.287.5457.1433

[8]	Arvidsson A, Collin T, Kirik D , et al. Neu-ronal replacement from endogenous precursors in the adult brain after stroke. Nat Med, 2002,8(9):963-970. doi: 10.1038/nm747 pmid: 12161747

[9]	Shmelkov S V, St C R, Lyden D , et al. AC133/CD133/Prominin-1. International Journal of Biochemistry & Cell Biology, 2005,37(37):715-719.

[10]	Coskun V, Wu H, Blanchi B , et al. From the cover: CD133 + neural stem cells in the ependyma of mammalian postnatal forebrain . Proceedings of the National Academy of Sciences of the United States of America, 2008,105(3):1026-1031. doi: 10.1073/pnas.0710000105

[11]	Beckervordersandforth R, Tripathi P, Ninkovic J , et al. In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells. Cell Stem Cell, 2010,7(6):744-758. doi: 10.1016/j.stem.2010.11.017 pmid: 21112568

[12]	Codega P, Silva-Vargas V, Paul A , et al. Prospective identification and purification of quiescent adult neural stem cells from their in vivo niche. Neuron, 2014,82(3):545-559. doi: 10.1016/j.neuron.2014.02.039 pmid: 24811379

[13]	Luo Y, Coskun V, Liang A , et al. Single-cell transcriptome analyses reveal signals to activate dormant neural stem cells. Cell, 2015,161(5):1175-1186. doi: 10.1016/j.cell.2015.04.001 pmid: 4851109

[14]	Hsieh J . Orchestrating transcriptional control of adult neurogenesis. Genes Dev, 2012,26(10):1010-1021. doi: 10.1101/gad.187336.112 pmid: 22588716

[15]	Bonaguidi M A, Wheeler M A, Shapiro J S , et al. In vivo clonal analysis reveals self-renewing and multipotent adult neural stem cell characteristics. Cell, 2011,145(7):1142-1155. doi: 10.1016/j.cell.2011.05.024 pmid: 3124562

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