The Structural and Functional Characteristics of Circular RNAs and Their Relationships with Gastric Cancer

doi:10.13523/j.cb.20180212

China Biotechnology

2018, Vol. 38

Issue (2): 82-88 DOI: 10.13523/j.cb.20180212

Orginal Article

The Structural and Functional Characteristics of Circular RNAs and Their Relationships with Gastric Cancer

Li-peng YAO^1,²,Wei GE¹,Ying-jun HU¹,Hai-yan LUO¹,Shan-shan WU¹,Fei-lei LIN¹,Jun-ming GUO^2*(

)

1 Ningbo College of Health Sciences,Ningbo 315000, China
2 Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology,Medical School of Ningbo University, Ningbo 315211, China

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Abstract

Circular RNAs (circRNAs) are a newly discovered RNA molecules, which are widely distributed in mammalian cells. They do not have 5'cap and 3' tail, but have a high degree of sequence conservation and stability, and not easy to be degraded by RNA exonuclease. What’s more, they not only can regulate the expression of genes, but also play a regulatory role at transcriptional or transcriptional levels. In recent years, circRNAs have been found to be associated with many tumors including gastric cancer. The formation, classification, molecular characterization and function of circRNAs were introduced. Then, the relationships between circRNAs and gastric cancer are analyzed from three aspects: the correlations between circRNAs and gastric cancer occurrence, the values of circRNAs in the diagnosis of gastric cancer, and the roles of circRNAs in the treatment of gastric cancer.

Key words： circRNA Gastric cancer Gene expression Tumor

Received: 30 October 2017 Published: 21 March 2018

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	Li-peng YAO
	Wei GE
	Ying-jun HU
	Hai-yan LUO
	Shan-shan WU
	Fei-lei LIN
	Jun-ming GUO

Cite this article:

Li-peng YAO,Wei GE,Ying-jun HU,Hai-yan LUO,Shan-shan WU,Fei-lei LIN,Jun-ming GUO. The Structural and Functional Characteristics of Circular RNAs and Their Relationships with Gastric Cancer. China Biotechnology, 2018, 38(2): 82-88.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180212 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I2/82

Fig.1 Models of circRNA formation

Table 1 Numbers of identified circRNAs in human cells

Fig.2 The main functions of circRNAsFAK: Focal adhesion kinase;HIF1α: Human hypoxia-inducible factor 1α; Tat: Transactivating regulatory protein; RCA: Rolling circle amplification mechanism; HDV: Hepatitis D virus; ci-ankrd52: ci-ankyrin repeat domain 52

circRNA	Sample type	Sample number	Expression in tumors	Clinical correlation	Function	Methods	Reference
hsa_circ_ 002059	Tissue/ Plasma	101 Paired cancer tissues and adjacent nontumorous tissues; 36 paired plasma samples from preoperative and postoperative patients	Down	Distal metastasis, TNM stage, Gender and age		qRT-PCR	[37]
hsa_circ_ 0001649	Tissue/ plasma	76 Tumor tissue samples and their paired paracancerous histological normal tissues; 20 patients’ whole blood samples preoperatively and postoperatively	Down	Pathological differentiation		qRT-PCR	[32]
hsa_circ_ 0000190	Tissue/ plasma	104 paired cancer tissues and adjacent non-tumor tissues; 104 plasma samples from cancer patients or health controls	Down	Tumor diameter, lymphatic metastasis,distal metastasis, TNM stage and CA19-9 levels		qRT-PCR	[33]
hsa_circ_ 0000096	Tissue	101 paired cancer tissues and adjacent non-tumorous tissues	Down		Promotes cancer cell growth and migration by regulating cyclin D1, CDK6, MMP-2 and MMP-9	qRT-PCR	[34]
circPVT1	Tissue	187 Paired cancer specimens and adjacent normal tissues	Up	An independent prognostic marker for overall survival and disease-free survival	Promote cell proliferation by acting as a sponge for miR-125 family members	qRT-PCR	[43]
hsa_circ_ 0003159	Tissue	108 paired gastric cancer tissues and adjacent non- tumorous tissues	Down	Gender, distal metastasis,tumor-node-metastasis stage		qRT-PCR	[46]
hsa_circ_ 0001895	Tissue	96 gastric cancer tissues and their adjacent non-tumorous tissues,35 healthy gastric mucosa and 30 gastric dysplasia tissues	Down	Cell differentiation, Borrmann type, tissue carcinoembryonic antigen expression		qRT-PCR	[47]
hsa_circ_ 0006633	Tissue/ plasma	96 gastric cancer tissues and their adjacent non-tumorous tissues,35 human healthy gastric mucosa, 51 gastritis mucosa, and 20 gastric dysplasia tissues;Plasma samples were obtained from 20 healthy volunteers and 20 gastric cancer patients	Down	Distal metastasis,tissue CEA level		qRT-PCR	[48]

Table 2 circRNAs in gastric cancer


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