γPNA——A New Type of High Efficient Peptide Nucleic Acid

doi:10.13523/j.cb.20180211

China Biotechnology

2018, Vol. 38

Issue (2): 75-81 DOI: 10.13523/j.cb.20180211

Orginal Article

γPNA——A New Type of High Efficient Peptide Nucleic Acid

Hao QIU,Ming-shu WANG,An-chun CHENG(

)

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University Institute of Preventive Veterinary Medicine Sichuan Agricultural University Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China

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Abstract

Peptide nucleic acid (PNA) is a synthetic analogue of DNA with the pseudopeptide backbone, which has the advantages of strong binding specificity with nucleic acid, high biological stability in tissues and cells and long half-life.By targeting DNA/RNA binding, PNA could inhibit the replication, transcription and translation of DNA/RNA.Chiral functional groups were introduced at gamma sites of the skeleton structure of PNA to form right-handed helical structures, which significantly improved the hybridization with targeting DNA/RNA, and this PNA derivative was called gamma PNA.The solubility, thermal stability and specificity of gamma PNA have been improved remarkably, and it has a good prospect in gene editing and probe detection. The structure, properties and research progress of gamma PNA are summarized, which provides theoretical basis and references for antisense PNA application.

Key words： γPNA; Gene regulation Probe detection Antisense application

Received: 11 July 2017 Published: 21 March 2018

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Cite this article:

Hao QIU,Ming-shu WANG,An-chun CHENG. γPNA——A New Type of High Efficient Peptide Nucleic Acid. China Biotechnology, 2018, 38(2): 75-81.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180211 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I2/75

Fig.1 Molecular structure and helix structure of γPNA(a) γPNA (b) DNA (c) Right handed helix (d) Left handed helix

Fig.2 Structure of γPNA and DNA hybrid complexes(a) γPNA-DNA (b) γPNA-DNA₂ (c) γPNA₂·DNA-DNA (d) γPNA₂-DNA₂

Fig.3 Regulation of translation by γPNA


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