Self-assembling Peptide R2I4R2 for Skin Wounds Repairing

doi:10.13523/j.cb.20180202

China Biotechnology

2018, Vol. 38

Issue (2): 7-12 DOI: 10.13523/j.cb.20180202

Orginal Article

Self-assembling Peptide R₂I₄R₂ for Skin Wounds Repairing

Hui-nan ZHANG,Meng-meng LI,Jing WEN,Shu-yi WU,Shi-jian LAN,Zhong-li LUO(

)

The College of Basic Medical Sciences, Molecular Medicine and Cancer Research Center,Chongqing Medical University, Chongqing 400016, China

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Abstract

The aim is proposed to understand the application of self-assembling peptide R₂I₄R₂ 3D cell culture human skin fibroblast in vitro and the role of the wound healing process. Circular dichroism was used to analysis its secondary structure influenced by different time,temperature and ion environment; Congo red staining was used to obtain the macro change of R₂I₄R₂ hydrogel; R₂I₄R₂ was used to 3D cell culture material to get the growth state of human skin fibroblast cell strain; AO / EB staining was used to study cell apoptosis in 3D culture environment;SD rat skin wound healing model was founded to check pathological changes by HE staining and immunohistochemical staining. These results indicated that R₂I₄R₂ can form a stable secondary structure in some conditions;24 hours after self-assembling, R₂I₄R₂ can form a homogeneous and stable membrane-like structure for 3D cell culture; Human skin fibroblasts had a hearty growing situation in hydrogel; The data in animal experiments show that R₂I₄R₂ can reduce inflammation, promote neovascularization, accelerate the skin wound repair process. It demonstrate this short peptide can be used to a new material in 3D cell culture and skin trauma repairing and beyond.

Key words： Self-assembling peptide Skin wounds repairing 3D cell culture

Received: 11 October 2017 Published: 21 March 2018

ZTFLH:

R318.08

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	Hui-nan ZHANG
	Meng-meng LI
	Jing WEN
	Shu-yi WU
	Shi-jian LAN
	Zhong-li LUO

Cite this article:

Hui-nan ZHANG,Meng-meng LI,Jing WEN,Shu-yi WU,Shi-jian LAN,Zhong-li LUO. Self-assembling Peptide R₂I₄R₂ for Skin Wounds Repairing. China Biotechnology, 2018, 38(2): 7-12.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20180202 OR https://manu60.magtech.com.cn/biotech/Y2018/V38/I2/7

Fig.1 Self-assembling time influence on the secondary structure of R₂I₄R₂by PBS(peptide concentration:0.1mmol/L) after 0h, 4h, 6h,8h, 22h

Fig.2 Temperature influence on the secondary structure of R₂I₄R₂Peptide concentration were 0.2mmol/L dissolved in PBS

Fig.3 Various ions influence on the secondary structure of R₂I₄R₂at 25 ℃ cultured in difference salt solution:0.15mmol/L NaCl,15mmol/L MgCl₂, PBS(pH=7.2), peptide concentration 0.1mmol/L

Fig.4 Congo red images of self-assembling peptide R₂I₄R₂

Fig.5 HFF-1 culture in 3D microenvironment of R₂I₄R₂

Fig.6 Image of AO/EB staining of HFF-1 in 3D microenvironment of R₂I₄R₂

Fig.7 Image of cck-8 of HFF-1 in 3D microenvironment of R₂I₄R₂

Fig.8 Image of wound area recovery in SD rats skin wound model

Fig.9 Image of HE staining of skin sound healing in peptide R₂I₄R₂

Fig.10 Image of Immunohistochemical staining of skin sound healing in peptide R₂I₄R₂


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