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| The Interaction Study Between Human Grx3 Protein and Transcription Factor p65 |
| HU Ke-yue1, CHENG Ning-hui2, WANG Xin-quan1 |
| School of Life Sciences, Tsinghua University, Beijing 100084, China |
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Abstract Oxidation-reduction system exists in almost all organisms,and thiol-redox enzymes play a very important part in the oxidation-reduction system. Thiol-redox enzymes regulate the conversion between thiol group and disulfide bond. Thiol-redox enzymes exist in both glutaredoxins system and thioredoxins system in human body. Our target protein Grx3 is one of the most important role in glutaredoxins system. p65(RELA) a very important protein in human body which has a very close relation with inflammatory reaction regulates the NF-κB signal pathway,it almost control all of the cascade reaction of NF-κB signal pathway,while other studies has shown that Grx3 has a significant influence on NF-κB signal pathway. There is a connection between p65 and Grx3 in vivo environment by colocalisationexperiment and p65 and Grx3 protein purify from E.coli can crosslink in a specific in vitro environment which provide a significant proof of interaction between Grx3 and p65 in vitro environment and explains how Grx3 get involved in the NF-κB signal pathway as while.
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Received: 03 March 2017
Published: 25 September 2017
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