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| 3D Hydrogel Compression Model and Effect of Cyclic Compression on Osteoblast Differentiation |
| ZHANG Ling-li, TONG Xiao-yang, GUO Jian-min, LEI Le, ZOU Jun |
| Shanghai University of Sport, Shanghai 200438, China |
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Abstract Objective:3D hydrogel cell model was established, and cyclic compressive loading on MC3T3-E1 cell with different intensities, frequencies and durations was applied, in order to research the suitable solution about promoting the osteoblast differentiation with cyclic compression. Methods:Cyclic compressive loading on MC3T3-E1 cell was applied with different intensity, frequency and time. After compressive loading finished, the total RNA extraction from cell-gel constructs were performed and quantified ATF4, ALP, Runx2, Osteocalcin, RANKL and RANK mRNA. Results:RANKL and RANK mRNA expression significantly with different frequencies cyclic compressive loading (P<0.05), and ALP mRNA (P<0.05) and Runx2 mRNA (P<0.01) expression significantly with different intensities and frequencies cyclic compressive loading (P<0.05). Meanwhile, Runx2 mRNA expression with 4h significant higher than 12h (P<0.05), and RANKL mRNA expression with 4h significant lower than 12h (P<0.05). Conclusion:Determine the stress intensity and frequency, 1% intensity, frequency of 0.5 Hz, 4 h of cyclic compression intervention could promote the growth of osteoblasts-like cells in the 3D hydrogel model.
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Received: 21 December 2016
Published: 25 August 2017
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