The Location of GFP Fused PHPT1 and Its Effect on Lamellipodia Formation

doi:10.13523/j.cb.20170603

China Biotechnology

2017, Vol. 37

Issue (6): 17-21 DOI: 10.13523/j.cb.20170603

The Location of GFP Fused PHPT1 and Its Effect on Lamellipodia Formation

XU An-jian¹, LI Yan-meng¹, WU Shan-na¹, LI Si-wen², ZHANG Bei¹, HUANG Jian¹

1. Experimental Center, Beijing Friendship Hospital, Beijing 100875, China;
2. Clinical Laboratory Center, Beijing Friendship Hospital, Beijing 100875, China

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Abstract Objective:To amplify human PHPT1 gene, and construct the C-terminal GFP fused vectors, investigate the expression and location of the fusion proteins in cells, as well as the relationship with lamellipodia. Methods:The cDNA sequence of PHPT1 gene was amplified and subcloned into pEGFP-N2 vectors. Transfecting of those vectors into HeLa cells to investigating the location statue of PHPT1 using Laser scanning confocal microscope, and further studied the relationship of PHPT1 location with lamellipodia formation. Results:The pEGFP-N2-PHPT1 prokaryotic expression vectors was constructed successfully and the location of PHPT1 in lamellipodia was closely related to lamellipodia formation. Conclusion:C-terminal GFP fused PHPT1 were expressed in the nucleus and the cytoplasm, especially in the leading edge of lamellipodia, and this location was closely related to lamellipodia formation and cell mobility.

Key words： Lamellipodia Location analysis PHPT1 GFP fused expression

Received: 22 December 2016 Published: 25 June 2017

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XU An-jian, LI Yan-meng, WU Shan-na, LI Si-wen, ZHANG Bei, HUANG Jian. The Location of GFP Fused PHPT1 and Its Effect on Lamellipodia Formation. China Biotechnology, 2017, 37(6): 17-21.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170603 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I6/17

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