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中国生物工程杂志

China Biotechnology
China Biotechnology  2017, Vol. 37 Issue (5): 45-51    DOI: 10.13523/j.cb.20170506
    
The Study of Protein-protein Interactions Using a Flow Cytometry-based FRET Technology in Living Cells
MENG Kun, HE Qing-yu, WANG Tong, LU Shao-hua
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Science and Technology, Jinan University, Guangzhou 510632, China
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Abstract  

Fluorescence resonance energy transfer (FRET) microscopy is widely used for the research of protein interactions in living cells. With the development of fluorescence activated cell sorting (FACS), FACS-based FRET not only could be well suited to study protein interactions in living cells but also could be measure and quantify FRET. However, may be due to the reason of flow cytometry is expensive and the special requirements of luminescent spectra by FRET, this technology was so far only applied to a few special scientific questions. To overcome these limitations developed a new FRET pair EGFP-mCherry and try to detect the FRET signal in the Accuri C6 flow cytometry, and then verify that the new FRET pair EGFP-mCherry was working by using two proteins p53 and MDM2 protein which there is an interaction has been clear. A new FERT pair EGFP-mCherry offers the precious possibility to detect FRET signal by using the Accuri C6 flow cytometry be successfully constructed, and not only promote the development of the FACS-FRET technique but also is expected to contribute to the identification of novel therapeutic target for treatment of human diseases.



Key wordsLiving cells      Fluorescence resonance energy transfer (FRET)      Protein interactions      Fluorescence activated cell sorting (FACS)     
Received: 10 October 2016      Published: 25 May 2017
ZTFLH:  Q51  
Cite this article:

MENG Kun, HE Qing-yu, WANG Tong, LU Shao-hua. The Study of Protein-protein Interactions Using a Flow Cytometry-based FRET Technology in Living Cells. China Biotechnology, 2017, 37(5): 45-51.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170506     OR     https://manu60.magtech.com.cn/biotech/Y2017/V37/I5/45

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