Synthesis of the ComX Pheromone Precursor Peptide in <em>Bacillus amyloliquefaciens</em> Q-426 Quorum Sensing System

doi:10.13523/j.cb.20170505

China Biotechnology

2017, Vol. 37

Issue (5): 38-44 DOI: 10.13523/j.cb.20170505

Synthesis of the ComX Pheromone Precursor Peptide in Bacillus amyloliquefaciens Q-426 Quorum Sensing System

WU Yi¹, ZHANG Li-ying¹, QUAN Chun-shan¹, ZHONG Mei-ling¹, WANG Lu-lu², WANG Guan-tian¹

1. Key Laboratory of the State Ethnic Affairs Commission-Ministry of Education, School of Life Science, Dalian Nationalities University, Dalian 116600, China;
2. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

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Abstract

The ComX pheromone precursor peptide of Bacillus amyloliquefaciens Q-426 quorum sensing system was successfully synthesized by Fmoc solid-phase synthesis method, and then isolated and purified by semi preparative chromatography (RP-HPLC) and liquid chromatography (HPLC-MS). A new method for the determination of free amino groups was established, and the effects of swelling process and reaction temperature on the degree of substitution were also investigated. The results showed that the traditional Kaiser reagent for the detection of free amino Fmoc in solid-phase synthesis can be replaced by the NNA reagent (ninhydrin + N-butanol + acetic acid solution). And the resins have the highest substitution degree after they were alternate swelling by DCM and DMF for 1h at 35℃. The product yield and the purity of the crude precursor peptide of ComX pheromone is 98% and 58.60% respectively. And the purity of the precursor peptide of ComX pheromone is 99% after being purified by semi preparative high performance liquid. Antibacterial experiments showed that the precursor peptide of ComX pheromone had obvious biological activity.

Key words： Fmoc solid phase synthesis Detection of amino Separation and purification The precursor peptide of ComX pheromone

Received: 11 November 2016 Published: 25 May 2017

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Cite this article:

WU Yi, ZHANG Li-ying, QUAN Chun-shan, ZHONG Mei-ling, WANG Lu-lu, WANG Guan-tian. Synthesis of the ComX Pheromone Precursor Peptide in Bacillus amyloliquefaciens Q-426 Quorum Sensing System. China Biotechnology, 2017, 37(5): 38-44.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170505 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I5/38

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