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| Hmox1 Can Promote BMP9-induced Osteogenesis in C3H10 T1/2 |
| LIU Xiao-hua, JI Cai-xia, XU Li, DONG Chao-qun, LUO Jin-yong |
| Key laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective:To study the effect of Hmox1 on BMP9-induced osteogenesis in C3H10T1/2. Methods:First of all, C3H10T1/2 were treated with Ad-BMP9, then the transcription and translation level of Hmox1 in C3H10T1/2 were detected with Q-PCR and Western blot. Subsequently, C3H10T1/2 were treated with Hmox1 agonist COPP and BMP9 or treated with Ad-Hmox1 or/and BMP9, ALP activity was detected by quantitative and staining assay at 5 day, Calcium deposition was assayed by Alizarin Red Staining at 14 day. At last, expression of COL1A1 was measured by Western blot. Results:C3H10T1/2 infected by Ad-BMP9 results in elevated gene and protein level of Hmox1. Ad-Hmox1 and Hmox1 agonist could enhance BMP9-induced ALP activity in C3H10T1/2. The expression of COL1A1 and calcium deposition were increased by Hmox1. Conclusion:Hmox1 can promote BMP9-induced osteogenic differentiation in C3H10T1/2.
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Received: 13 September 2016
Published: 25 April 2017
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