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| Runx1 Enhances BMP9-induced Osteogenic Differentiation in Mesenchymal Stem Cell Line MEFs |
| JI Cai-xia, LIU Xiao-hua, XU Li, DONG Chao-qun, LUO Jin-yong |
| Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective:To investigate the role of Runx1 gene on BMP9-incuced osteogenic differentiation of Mouse embryonic fibroblasts (MEFs). Methods:MEFs were infected with Ad-BMP9 and then the expression of endogenous Runx1 at the mRNA and protein level was determined by RT-PCR and Western blot.The recombinant adenovirus Ad-Runx1 was constructed and its expression was validated at the mRNA and protein level. MEFs cells were treated with Ad-Runx1 or/and BMP9, the ALP activity was detected by quantitative and staining assay, calcium deposition was detected by Alizarin Red S staining. Runx2, as a core osteogenic transcription factor, was detected by RT-PCR and Western blot at the mRNA and protein level. Result:The expression of Runx1 was up-regulated by BMP9 at the mRNA and protein level in MEFs cells; Ad-Runx1 could enhance the expression of Runx1 at the mRNA and protein level. Runx1 can increase ALP activity and calcium deposition of MEFs cells induced by BMP9,and promote then expressions of Runx2 at the mRNA and protein level. Conclusion:BMP9-induced osteogenic differentiation is partially improved by Runx1 in mesenchymal stem cell line MEFs.
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Received: 15 August 2016
Published: 25 March 2017
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