Selective Uptake and Increased Accumulation of Free Saturated Fatty Acids by the Yeast <em>Saccharomyces cerevisiae</em> and <em>Yarrowia lipolytica</em>

doi:10.13523/j.cb.20170210

China Biotechnology

2017, Vol. 37

Issue (2): 63-73 DOI: 10.13523/j.cb.20170210

Selective Uptake and Increased Accumulation of Free Saturated Fatty Acids by the Yeast Saccharomyces cerevisiae and Yarrowia lipolytica

WEI Xuan^1,2, HAO Ya-qiao^1,2, Susanna Leong Su Jan³, WU Yan², LIU Ye-fei¹, ZHAO Hong-xin²

1. College of Life Science Shenyang Normal University, Shenyang 110034, China;
2. Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;
3. School of Chemical and Biomedical Engineering, Nanyang Technological University 369798, Singapore

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Abstract

It has quite significance and broad application prospects to develop novel strategies to produce special functional fatty acids with high added value by oleaginous microorganisms. Two kinds of yeasts S. cerevisiae and Y. lipolytica were selected to test how to selective uptake and increased accumulation of free saturated fatty acids. The results shown that when C ≤ 10 carbon chain length,free saturated fatty acids couldn't be uptaked and utilized on the contrary to suppresses the growth of cell, especially C≤ 8 cells were killed quickly; When C = 11, it had certain inhibitory effect on the growth of cells which leads to the weak growth of bacteria; When C ≥12, it had no impact on cell growth and cells grew well; Even C fatty acids was digested faster than the base C fatty acids; Lipid body could be visualized by fluorescence microscopy of cells stained with Nile red. No significant variations were observed in S. cerevisiae or its mutants grown on YNBD. Where only a few small LB were observed in Y. lipolytica grown on YNBD. After growth YNBF medium, Y. lipolytica strain contained several larger LB and a few smaller ones in cell; The experiment of GC/Mass analysis indicated that the most of fatty acids except long-chain fatty acid (C≥16) could be uptaked and accumulated by S. cerevisiae S228C BY4741-pox1 or S. cerevisiae S228C BY4741-pox1, 3 from culture medium, however medium-chain fatty acid couldn't be accumulated by original S. cerevisiae S228C BY4741. Thus, yeast could uptake and accumulate saturated fatty acid based on different carbon chain length. The most of fatty acids except long-chain fatty acid (C≥16) could be uptaked and accumulated by S. cerevisiae S228C BY4741-pox1 or S. cerevisiae S228C BY4741-pox1, 3 mutants from culture medium. The most of fatty acids also could be uptaked by Y. lipolytica from culture medium in cell.

Key words： Free fatty acids Oleaginous yeast Toxicity Microbial oils

Received: 21 July 2016 Published: 25 February 2017

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WEI Xuan, HAO Ya-qiao, Susanna Leong Su Jan, WU Yan, LIU Ye-fei, ZHAO Hong-xin. Selective Uptake and Increased Accumulation of Free Saturated Fatty Acids by the Yeast Saccharomyces cerevisiae and Yarrowia lipolytica. China Biotechnology, 2017, 37(2): 63-73.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20170210 OR https://manu60.magtech.com.cn/biotech/Y2017/V37/I2/63

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