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| Effects of S100A6 on Proliferation and Migration of Human Cervical Cancer Cells and Its Mechanism |
| LI Ai-fang, GU Yue, LI Xue-ru, SUN Hui, ZHA He, XIE Jia-qing, ZHAO Jia-li, ZHOU Lan |
| Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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Abstract Objective: To investigate the effects of S100A6 on cell proliferation and migration of cervical cancer cells and explore its mechanism. Methods: Quantitative polymerase chain reaction (qPCR) was used to detect the basic expression of S100A6 in HeLa, SiHa and CaSki cells; HeLa and SiHa cells were treated with recombinant adenoviruses AdS100A6 and AdsiS100A6 to upregulate and downregulate S100A6 expression respectively, Western blot was used to verify the effects of virus infection. MTT method was used to detect cell proliferation ability; wound healing assay was used to detect cell migration ability; Western blot was used to detect the level of E-cadherin, N-cadherin and p-Akt; reverse transcription and polymerase chain reaction(RT-PCR) was used to detect the level of Snail and Twist, the downstream target genes of PI3K-Akt pathway. Results: In AdS100A6 group (HeLa cells), the OD492 value and wound healing rate at the 3rd day were increased, N-cadherin was increased, but E-cadherin was decreased; In AdsiS100A6 group (SiHa cells), the OD492 value at the 5th day and wound healing rate at the 3rd day were decreased, N-cadherin was decreased, but E-cadherin was increased; At the same time, in AdS100A6 group, p-Akt was increased, Snail and Twist were also increased obviously. Conclusion: S100A6 promotes cervical cancer cells proliferation and migration and the effect may be mediated by inducing epithelial-mesenchymal transition and activating PI3K/Akt signaling pathway.
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Received: 28 July 2016
Published: 25 February 2017
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