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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (11): 16-22    DOI: 10.13523/j.cb.20161103
    
Effects of Lipid Production by Rhodosporidium toruloides under Conditions with Limitation of Different Nutrient Elements
WANG Ya nan1,2, SHEN Hong wei1, YANG Xiao bing1, ZHAO Zong bao1
1. Division of Biotechnology and Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, Dalian 116023, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
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Abstract  

To compare lipid production among different nutrient-limited conditions, the oleaginous yeast Rhodosporidium toruloides was cultivated on glucose with limitation of nitrogen-or phosphorus-or sulfur-sources on the flasks. Then, the lipid accumulation on 3-liter bioreactors by varying carbon concentrations at the C/P of 1 133.3 were surveyed. Our results demonstrated that lipid content of over 60% could be achieved by limiting either one of these three elements. By controlling the recipe of culture media, it was possible to prepare lipids of which contents of saturated fatty acids were over 70% or contents of unsaturated fatty acids were over 60%. P-limitation was more effective comparing to N-limitation or S-limitation for higher lipid yield and productivity. The information is valuable for microbial lipid technology using different biological resources as feedstock.



Key wordsOleaginous yeast      Nutrient limitation      Rhodosporidium toruloides      Microbial lipids     
Received: 23 May 2016      Published: 25 November 2016
ZTFLH:  Q93-33  
Cite this article:

WANG Ya nan, SHEN Hong wei, YANG Xiao bing, ZHAO Zong bao. Effects of Lipid Production by Rhodosporidium toruloides under Conditions with Limitation of Different Nutrient Elements. China Biotechnology, 2016, 36(11): 16-22.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20161103     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I11/16

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