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| Control of Acetone Concentration and Acetone/Butanol Ratio in ABE Fermentation by C. acetobutylicum with a Novel Glucose/Acetate Co-substrate System Incorporating Glucose Limitation |
| WANG Hao1, ZHANG Jing-shu2, DING Jian1, LUO Hong-zhen1, CHEN Rui1, SHI Zhong-ping1 |
1 Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;
2 China Shijiazhuang Pharmaceutical Group Company Limited, Shijiazhuang 050035, China |
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Abstract A novel strategy for arbitrarily controlling acetone concentration and acetone/butanol ratio in ABE fermentation by C. acetobutylicum was proposed. With this strategy, (1) the residual glucose concentration could reduce to low level close to 0 g/L when ABE fermentation enters the solventogenic phase, by adaptively controlling the initial glucose concentration in the corn-based medium via glucoamylase usage dose, reaction time and temperature regulation; (2) Under the condition of glucose limitation, C. acetobutylicum has the ability to release more glucoamylase for its survival and the secreted glucoamylase could continuously hydrolyze oligosaccharide to maintain glucose at low concentration, and therefore repress the metabolism or synthesis rates of glycolysis and NADH in C. acetobutylicum. At the same time, exogenous addition of acetate creates a glucose/acetate co-substrate environment. The co-substrate system would not deteriorate the energy metabolisms in ABE fermentation, but could adaptively repress butanol synthesis avoiding the early occurrence of butanol inhibition and enhance bio-acetone synthesis by effectively utilizing the exogenously added acetate; (3) On the top of exogenous acetate addition, adaptively adding certain amount of viable S. cerevisiae to form C. acetobutylicum/S. cerevisiae co-culturing system, would enhance C. acetobutylicum tolerant ability against higher butanol concentration environment. The entire system could arbitrarily control acetone concentration and acetone/butanol ratio in the ranges of 5~12 g/L and 0.5~1.0, their maximum values could reach levels of 11.74 g/L and 1.02, while maintaining butanol concentration within normal range of 10~14 g/L, to satisfy different requirements on acetone and butanol products in industrial ABE fermentation.
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Received: 18 April 2016
Published: 25 October 2016
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