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| Influence on the Conversion Efficiency of Induced Differentiation of Various Potato Tissues |
| ZHU Xue-rui1, JI Jing1, WANG Gang1, MA Zhi-gang2, YANG Dan1, JIN Chao1, LI Chen1 |
1. School of Environmental Science and Engineering, Tianjin University, Tianjing 300072, China;
2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract With rapid development of agriculture,genetic engineering as an important means of transformation of the potato traits has been extensively applied.Optimization of potato tissue culture system and exogenous gene conversion conditions are the basis of the work of the transgenic potato.Three potato cultivars having different maturity period(Dongnong 303,Zaodabai,Atlantic)have been tested, with the use of orthogonal experiment,screen,optimize stem segments and Microtuber differentiation system.Stem callus regeneration system and in vitro differentiation of potato direct regeneration system were built.Then applied genetic transformation with Agrobacterium tumefaciens including the reconstruction vector carrying the target gene LYCB and selection marker NPTII.Respectively,the three materials stem section plantlets and microtubers were manipulated,and the transgenic conversion conditions were optimized for each one.Then the three were applyied to the best regeneration system and genetic transformation system,finally positive plants using PCR screening and statistically analysised the data were got.The results show that conversion rate via stem callus of Dongnong 303,Zaodabai and Atlantic respectively were 36%,35%,28%;direct differentiation rate via Microtuber of them separately were 43%,45%,17%.In conclusion,microtuber of Dongnong 303/Zaodabai is suitable for the conversion tissue of test organization,stem of the Atlantic is better conversion one.
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Received: 03 May 2016
Published: 25 October 2016
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