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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (10): 8-14    DOI: 10.13523/j.cb.20161002
    
Genetic Analysis and Gene Mapping of Recessive Genic Male Sterility14 (ms14) Mutant in Maize
YU Zi-qing1,2, WU Suo-wei1,2, ZHANG Dan-feng1,2, LIU Shuang-shuang1,2, XIE Ke1,2, RAO Li-qun1, WAN Xiang-yuan1,2
1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;
2. Institute of Industrial Technology on Modern Bio-Agriculture, Beijing Shou Jia Li Hua Sci-Tech Co. Ltd., Beijing 100192, China
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Abstract  

Maize (Zea mays L.) genic male sterility mutant lines are one kind of elite germplasm, which is helpful to genetics and breeding research and hybrid production in maize. A maize genic male sterility mutant, male sterility14 (ms14) was preliminary studied. Firstly, phenotypical analysis and I2-KI dyeing of ms14 tassels and anthers showed that ms14 mutant is completely pollen sterile and exerts no anthers. Secondly, genetic analysis using two F2 segregation populations of ms14 (ms14×zheng58 and ms14×chang7-2), the ratio of fertile to sterile plants is 3 to 1, which shows that the ms14 mutant is controlled by a recessive genic male sterility gene. Thirdly, through the linkage analysis between ms14 locus and molecular markers in the F2 segregation populations of ms14 (ms14×chang7-2), the ms14 gene is roughly mapped to an interval on Chromosome 1 between SSR markers umc2025 and umc1676, with the genetic distance of 2.2 cM and 0.3 cM, respectively. Finally, the potential application value of the ms14 gene in maize hybrid breeding and production is discussed.

Key words： Recessive genic male sterility      Gene mapping      Zea mays      Genetic analysis      ms14     
Received: 02 March 2016      Published: 25 October 2016
ZTFLH:  Q785  
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Cite this article:

YU Zi-qing, WU Suo-wei, ZHANG Dan-feng, LIU Shuang-shuang, XIE Ke, RAO Li-qun, WAN Xiang-yuan. Genetic Analysis and Gene Mapping of Recessive Genic Male Sterility14 (ms14) Mutant in Maize. China Biotechnology, 2016, 36(10): 8-14.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20161002     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I10/8

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主管：中国科学院 　主办：中国科学院文献情报中心 　中国生物技术发展中心 　中国生物工程学会