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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (5): 125-131    DOI: 10.13523/j.cb.20160518
    
Advances in the Research of miRNA Promoters in Plants
CHEN Min, CHEN Hui, BAO Hai, HUANG Peng, WANG Yan-wei
National Engineering Laboratory for Tree Breeding, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
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Abstract  

MicroRNAs (miRNAs) are a class of 20~24 nucleotides (nt) non-coding small RNAs with wide distribution in plant genomes. As one of the most important post-transcriptional regulators, miRNAs play a key role in gene expression and stress responses of plants through cleavage or translational repression by complete or partial complementarity to target mRNAs. MIRNA genes are generally known to be transcribed in the nucleus by RNA polymerase II (Pol II) into a primary miRNA with two following continuous cleavages by Dicer-like 1 to produce a precursor miRNA and a miRNA:miRNA star duplex. Promoters are indispensable components involved in gene expression and contain transcription start sites (TSS), TATA boxes and upstream cis-acting elements. It is known that the promoters of miRNAs are important in regulating the expression of miRNA in specific plant tissues, development stages, biotic and abiotic stress responses of plants. As a consequence, investigations on promoters of MIRNA genes are fundamentally important to reveal the molecule mechanism of spatial and temporal expression of miRNAs and will be helpful to reveal the function of miRNAs involved in the biological processes of plants. Consequently, it is highly indispensable to review and analyze the characteristics of miRNA promoters, identification methods of miRNA core promoters and cis-acting elements. Furthermore, the advances of researches on miRNA promoters of plants such as Arabidopsis and rice, the possible problems and prospects of miRNA promoters investigations were also discussed to provide insights into the evaluation of miRNA-mediated gene regulation mechanism.



Key wordsPromoter      Cis-acting elements      miRNA      Plant     
Received: 11 December 2015      Published: 26 January 2016
ZTFLH:  Q78  
Cite this article:

CHEN Min, CHEN Hui, BAO Hai, HUANG Peng, WANG Yan-wei. Advances in the Research of miRNA Promoters in Plants. China Biotechnology, 2016, 36(5): 125-131.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160518     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I5/125

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