Biomaterial of [PEAD: Heparin: NGF] Coacervate Promote Function Recovery of Sciatic Nerve Regeneration in Rats

doi:10.13523/j.cb.20160210

China Biotechnology

2016, Vol. 36

Issue (2): 68-72 DOI: 10.13523/j.cb.20160210

Biomaterial of [PEAD: Heparin: NGF] Coacervate Promote Function Recovery of Sciatic Nerve Regeneration in Rats

LI Rui, CAI Ping-tao, YE Li-bing, ZHANG Hong-yu, XIAO Jian

College of Pharmacy of Wenzhou Medical University, Zhejiang Provincial Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou 325035, China

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Abstract

Purpose:To overcome the defect of nerve growth factor(NGF) in the treatment of sciatic nerve injury. A polycation-poly(argininylaspartate diglyceride ethylene)(PEAD) was designed to substitute the heparin-binding sequence of the FGF receptor and form a ternary complex containing the polycation, heparin and NGF, which provides experimental evidence for the clinical treatment of peripheral nerve injury. Methods:Experiment young adult male Wistar rats aged 8 weeks(200~220 g) were selected and divided randomly into 3 groups:Saline group, Free NGF group and NGF coacervate group with 8 rats in each group. The common sciatic nerve was exposed at the level of the middle of the thigh by blunt dissection through the biceps femoris and moderate contusion injuries were performed using a vascular clip, the skin was then closed with 5-0 stitches. Following surgery, each group was injected equivalent volume saline, free NGF(80 ng/day), or the NGF coacervate containing equivalent NGF in the injury sites of right limb through a 1 ml syringe. At 30 days after surgery, the regenerated rat sciatic nerve was evaluated by footprint analysis, immunohistochemistry and histologic assessment. Experimental data were processed using the statistical software SPSS 13. 0.Results:The behavior, pathological structure and protein expression treated by NGF or NGF coacervate exhibited better than that saline group. Moreover, the therapeutic effect of NGF coacervate group was better than free NGF group. Conclusion:The new type of coacervate loaded with NGF could evident effects on the repair and regeneration of peripheral nerve injury, this may lay the groundwork for future translational studies of NGF coacervate for peripherial nervous system(PNS) diseases, especially those related to sciatic nerve injury.

Key words： Sciatic nerve injury Heparin Nerve growth factor Coacervate

Received: 20 November 2015 Published: 14 December 2015

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LI Rui, CAI Ping-tao, YE Li-bing, ZHANG Hong-yu, XIAO Jian. Biomaterial of [PEAD: Heparin: NGF] Coacervate Promote Function Recovery of Sciatic Nerve Regeneration in Rats. China Biotechnology, 2016, 36(2): 68-72.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160210 OR https://manu60.magtech.com.cn/biotech/Y2016/V36/I2/68

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