Construction and Pathogenicity Analysis of ATMT Mutant of <em>Curvularia lunata</em>

doi:10.13523/j.cb.20160104

China Biotechnology

2016, Vol. 36

Issue (1): 23-28 DOI: 10.13523/j.cb.20160104

Construction and Pathogenicity Analysis of ATMT Mutant of Curvularia lunata

NI Xuan, JIANG Xue, LI Ya-qian, CHEN Jie

School of Agriculture and Biology, Shanghai Jiao Tong University, Key Laboratory of South Urban Agriculture, Ministry of Agriculture, Key Laboratory of Microbial Metabolism, Shanghai 200240, China

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Abstract

Agrobacterium-mediated transformation were used to construct the transformants of the Curvularia lunata and 1454 transformants were obtained. Then screened eight mutants in which colonial morphology, mycelial growth and the expression of disease related genes changed more distinct. Inoculating the mutants into the detached leaves has been used to screen mutant strains and found that 4 mutant strains whose pathogenicity reduced, and 2 mutant strains whose pathogenicity enhanced. Determining the growth rate, sporulation quantity and the activity of cell wall degradation enzymes showed that the sporulation quantity of the mutants whose pathogenicity reduced were all decreasd and one of the strains did not even produce a spore. The activity of the PG enzyme enhanced generally, but the changes of the Cx enzyme did not have differences apparently. However, the activity of the Cx enzyme of the two mutants whose pathogenicity enhanced was increased.

Key words： Pathogenicity ATMT Curvularia lunata

Received: 26 August 2015 Published: 11 January 2016

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Cite this article:

NI Xuan, JIANG Xue, LI Ya-qian, CHEN Jie. Construction and Pathogenicity Analysis of ATMT Mutant of Curvularia lunata. China Biotechnology, 2016, 36(1): 23-28.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160104 OR https://manu60.magtech.com.cn/biotech/Y2016/V36/I1/23

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