Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (12): 78-83    DOI: 10.13523/j.cb.20151212
    
Comparison of Seed Sludge Pretreatment Methods for Hydrogen Production from Sewage Sludge and Food Waste
WU Yun-qi, LI Ru-ying
School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China
Download: HTML   PDF(736KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Batch experiments are conducted to produce hydrogen from sewage sludge and food waste. Six commonly used hydrogen-producing seed sludge pretreatment methods are compared, including heat treatment, chemical inhibitor sodium 2-bromoethanesulphonate (BESA) treatment, acid treatment, alkali treatment, continuous aeration and repeated aeration. Results show that hydrogen yield of seed sludge without pretreatment is lowest, with hydrogen uptake and methane production. Hydrogen production by seed sludge pretreated by BESA, acid, continuous aeration and repeated aeration perform relatively better, among which repeated aeration treated sludge has highest hydrogen yield, with the value of 86.9 ml-H2/g-VSadded. These four pretreatment methods can obviously inhibit methanogens, but the other two pretreatment methods, heat and alkali treatments cannot inhibit methanogens effectively, with lower hydrogen yields and hydrogen uptake as well as methane production. The pH drop during hydrogen production also inhibits methane production activity.



Key wordsFermentative hydrogen production      Sewage sludge      Seed sludge pretreatment      Food waste     
Received: 21 July 2015      Published: 22 December 2015
ZTFLH:  Q819  
Cite this article:

WU Yun-qi, LI Ru-ying. Comparison of Seed Sludge Pretreatment Methods for Hydrogen Production from Sewage Sludge and Food Waste. China Biotechnology, 2015, 35(12): 78-83.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20151212     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I12/78

[1] Prawit K, Booki M, Irini A. Biohydrogen production from xylose at extreme thermophilic temperatures(70℃) by mixed culture fermentation. Water Research, 2009, 43(5):1414.
[2] Bita B, George N, Dimitre K, et al. The effect of heat pretreatment temperature on fermentative hydrogen production using mixed cultures. International Journal of Hydrogen Energy, 2008, 33(15):4064.
[3] Jia X, Zhu C W, Li M X, et al. A comparison of treatment techniques to enhance fermentative hydrogen production from piggery anaerobic digested residues. International Journal of Hydrogen Energy, 2013,38:8691-8698.
[4] Wang J L, Wei W. Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge. International Journal of Hydrogen Energy, 2008, 33(12):2834-2941.
[5] Yokoi H, Saitsu A S, Uchida H, et al. Microbial hydrogen production from sweet potato starch residue. Journal of Bioscience and Bioenergy, 2001, 91:58-63.
[6] Collet C, Adler N, Schwitzguebel J P, et al. Hydrogen production by Clostridium thermolacticum during continuous fermentation of lactose. International Journal of Hydrogen Energy, 2004, 29:1479-1485.
[7] Liu G, Shen J. Effects of culture medium and medium conditions on hydrogen production from starch using anaerobic bacteria. Journal of Bioscience and Bioenergy, 2004, 98:251-256.
[8] Lin C Y, Lay C H. Carbon/nitrogen ratio effect on fermentative hydrogen production by mixed microflora. International Journal of Hydrogen Energy, 2004, 29:41-45.
[9] Evvyernie D, Morimoto K, Karita S, et al. Conversion of chitinous waste to hydrogen gas by Clostridium paraputrificum M-21. Journal of Bioscience and Bioenergy, 2001, 91:339-343.
[10] Wang C C, Chang C W, Chu C P, et al. Producing hydrogen from wastewater sludge by Clostridum bifermentans. Journal of Biotechnology, 2003, 102:83-92.
[11] Kenealy W, Zeikus J G. Influence of corrinoid antagonists on methanogen metabolism. Bacteriol, 1981,146:133-140.
[12] Zhu H G, Michel B. Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge. International Journal of Hydrogen Energy, 2006,31:1980-1988.
[13] Andrea G Valentina S, Maria C L, et al. Evaluation of aeration pretreatment to prepare an inoculum for the two-stage hydrogen and methane production process. Bioresource Technology, 2014,166:211-218.
[14] 刘新媛.污泥和餐厨废物两相双温发酵产氢产甲烷研究.天津:天津大学,2014. Liu X Y. Hydrogen and methane production from co-digestion of sludge and food waste in a temperature-separated two-stage anaerobic fermentation process. Tianjin:Tianjin University, 2014.
[15] APHA. Standard methods for the examination of water and wastewater. 21st ed. Washington DC:APHA, 2005.
[16] Gaudy A F. Colorimetric determination of protein and carbohydrate. Industial Water Wastes, 1962, 7:17-22.
[17] Lowery O H, Rosebrough N J, Farr A L, et al. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 1951, 193(1):265-275.