| [1] Arterburn L M, Hall E, Boken H. Distribution, interconversion, and dose response of n-3 fatty acids in humans. American Journal of Clinical Nutrition, 2006, 83(6):1467S-1476S.
[2] 李会珍,张志军.植物合成长链多不饱和脂肪酸研究进展.中国生物工程杂志, 2008, 28(12):112-115. Li H Z, Zhang Z J. Progresses on biosynthesis of long chain polyunsaturated fatty acids in plant. China Biotechnology, 2008. 28(12):112-115.
[3] Hong H, Datla N, Reed D W, et al. High-level production of γ-linolenic acid in Brassica juncea using a Δ6 desaturase from Pythium irregulare. Plant Physiology, 2002, 129(1):354-362.
[4] Liu J, Li D, Yin Y, et al. Δ6-Desaturase from Mortierella alpina:cDNA cloning, expression, and phylogenetic analysis. Biotechnology Letters, 2011, 33(10):1985-1991.
[5] 黎志勇,纪晓俊,丛蕾蕾,等.发酵法生产亚麻酸的研究进展.中国生物工程杂志, 2010, 30:110-117. Li Z Y, Ji X J, Cong L L, et al. Progresses in fermentative production of γ-linolenic acid. China Biotechnology, 2010, 30:110-117.
[6] Xue Z, Sharpe P L, Hong S P, et al. Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature Biotechnology, 2013,31(8):734-740.
[7] Grima E M, Medina A R, Giménez A G, et al. Gram-scale purification of eicosapentaenoic acid (EPA, 20:5n-3) from wet Phaeodactylum tricornutum UTEX 640 biomass. Journal of Applied Phycology, 1996, 8(4-5):359-367.
[8] Liu Y, Tang J, Li J J, et al. Efficient production of triacylglycerols rich in docosahexaenoic acid (DHA) by osmo-heterotrophic marine protists. Applied Microbiology and Biotechnology, 2014, 98(23):9643-9652.
[9] Hao G, Chen H, Du K, et al. Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina. Biotechnology Letters, 2014, 36(9):1827-1834.
[10] Sakuradani E, Ando A, Shimizu S, et al. Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4. Journal of Bioscience and Bioengineering, 2013, 116(4):417-422.
[11] Sakuradani E, Kobayashi Mshimizu S. Δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus:Gene cloning and its heterologous expression in a fungus, Aspergillus. Gene, 1999, 238(2):445-453.
[12] Sakuradani E, Shimizu S. Gene cloning and functional analysis of a second delta 6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus. Bioscience, Biotechnology, and Biochemistry, 2003, 67(4):704-711.
[13] 代鹏,陈海琴,顾震南,等.高山被孢霉生产多不饱和脂肪酸发酵条件的研究进展.食品工业科技, 2014, 35(5):354-359. Dai P, Chen H Q, Gu Z N. Research progress in fermentation condition for polyunsaturated fatty acids by Mortierella Alpina. Science and Technology of Food Industry, 2014, 35(5):354-359.
[14] Zhang Q, Li M, Ma H, et al. Identification and characterization of a novel Δ6-fatty acid desaturase gene from Rhizopus arrhizus. FEBS Letters, 2004, 556(1):81-85.
[15] Zhou X R, Robert S, Singh S, et al. Heterologous production of GLA and SDA by expression of an Echium plantagineum delta 6-desaturase gene. Plant Science, 2007, 172(2):421-422.
[16] 丁兆坤,麻艳群,许友卿.合成高度不饱和脂肪酸去饱和酶的分子生物学研究Ⅱ.克隆,表达与功能分析.中国生物工程杂志, 2008, 28:196-200. Ding Z K, Ma Y Q, Xu Y Q. Studies on molecular biology of the desaturases of highly unsaturated fatty acid biosynthesis-Ⅱ. Cloning, expression and function analysis. China Biotechnology, 2008, 28:196-200.
[17] Wang L, Chen W, Feng Y, et al. Genome characterization of the oleaginous fungus Mortierella alpina. PloS one, 2011, 6(12):e28319.
[18] Hsiao T Y, Holmes B, Blanch H W. Identification and functional analysis of a delta-6 desaturase from the marine microalga Glossomastix chrysoplasta. Marine Biotechnology, 2007, 9(2):154-165.
[19] Iskandarov U, Khozin-Goldberg I, Cohen Z. Identification and characterization of Δ12, Δ6, and Δ5 desaturases from the green microalga Parietochloris incisa. Lipids, 2010, 45(6):519-530.
[20] Kajikawa M, Yamato K T, Kohzu Y, et al. Isolation and characterization of Δ6-desaturase, an ELO-like enzyme and Δ5-desaturase from the liverwort Marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Plant Molecular Biology, 2004, 54(3):335-352.
[21] Kim S H, Kim J B, Kim S Y, et al. Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli). Biotechnology Letters, 2011, 33(6):1185-1193.
[22] Ma X, Yu J, Zhu B, et al. Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata. Chinese Journal of Oceanology and Limnology, 2011, 29:290-296.
[23] Zahringer U, Domergue F, Abbadi A, et al. In vivo characterization of the first acyl-CoA delta6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri. Biochem J, 2005, 389:483-490.
[24] 奥斯伯.精编分子生物学实验指南.第5版.北京:科学出版社, 2008.545-581. Ausubel F. Short Protocols in Molecular Biology. Fifth ed. Beijing:Science Press, 2008.545-581.
[25] Zhang B, Rong C, Chen H, et al. De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica. Microb Cell Fact, 2012, 11(1):51. |
