| [1] Hu Q, Sommerfeld M, Jarvis E, et al. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J, 2008, 54(4): 621-639.
[2] Chisti Y. Biodiesel from microalgae. Biotechnol Adv, 2007, 25(3): 294-306.
[3] Breuer G, Lamers P P, Martens D E, et al. The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresour Technol, 2012, 124(337): 217-226.
[4] Beermann C, Green A, Möbius M, et al. Lipid class separation by HPLC combined with GC FA analysis: comparison of seed lipid compositions from different Brassica napus L. varieties. J Am Oil Chem Soc, 2003, 80(8): 747-753.
[5] Ruiz-Gutiérrez V, Pérez-Camino M C. Update on solid-phase extraction for the analysis of lipid classes and related compounds. J Chromatogr A, 2000, 885(1-2): 321-341.
[6] Chen W, Sommerfeld M, Hu Q. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae. Bioresour Technol, 2011,102(1): 135-141.
[7] Isleten-Hosoglu M, Gultepe I, Elibol M. Optimization of carbon and nitrogen sources for C biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content. Biochem Eng J, 2012, 61: 11-19.
[8] Romek M, Barbara G, Ewa K, et al. New technique to quantify the lipid composition of lipid droplets in porcine oocytes and pre-implantation embryos using Nile Red fluorescent probe. Theriogenology, 2011, 75(1): 42-54.
[9] Gao C F, Wang Y, Shen Y, et al. Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes. BMC Genomics, 2014, 15: 582-595.
[10] Fan J H, Cui Y B, Wan M X, et al. Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors. Biotechnol Biofuels, 2014, 7:17-30.
[11] Lin J T. HPLC separation of acyl lipid classes. J Liq Chromatogr Relat Technol, 2007, 30(13-16): 2005-2020.
[12] Rodríguez-Alcalá L M, Fontecha J. Major lipid classes separation of buttermilk, and cows, goats and ewes milk by high performance liquid chromatography with an evaporative light scattering detector focused on the phospholipid fraction. J Chromatogr A, 2010, 1217(18): 3063-3066.
[13] McLaren D G, Miller P L, Lassman M E, et al. An ultraperformance liquid chromatography method for the normal-phase separation of lipids. Anal Biochem, 2011, 414(2): 266-272.
[14] Donot F, Cazals G, Gunata Z, et al. Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS. J Chromatogr B, 2013,942-943(24): 98-106.
[15] Wang H T, Yao C H, Ai J N, et al. Identification of carbohydrates as the major carbon sink of the marine microalga Isochrysis zhangjiangensis (Haptophyta) and optimization of its productivity by nitrogen manipulation. Bioresour Technol, 2014, 171: 298-304.
[16] Wang H T, Meng Y Y, Cao X P, et al. Coordinated response of photosynthesis, carbon assimilation, and triacylglycerol accumulation to nitrogen starvation in the marine microalgae Isochrysis zhangjiangensis (Haptophyta). Bioresour Technol, 2015, 177(12): 282-288.
[17] Hibberd D J. Notes on taxonomy and nomenclature of the algal classes Eustigmatophyceae and Tribophyceae (synonym Xanthophyceae). Bot J Linn Soc, 1981, 82(2): 93-119.
[18] Campos H, Boeing W J, Dungan B N, et al. Cultivating the marine microalga Nannochloropsis salina under various nitrogen sources: effect on biovolume yields, lipid content and composition, and invasive organisms. Biomass Bioenergy, 2014, 66(7): 301-307.
[19] Rodolfi L, Zittelli G C, Bassi N, et al. Microalgae for oil: strain selection, induction of lpid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng, 2009, 102(1): 100-112.
[20] Van Vooren G, Le Grand F, Legrand J, et al. Investigation of fatty acids accumulation in Nannochloropsis oculata for biodiesel application. Bioresour Technol, 2012, 124(3): 421-432.
[21] Xiao Y, Zhang J T, Cui J T, et al. Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress. Bioresour Technol, 2013, 130: 731-738.
[22] Meng Y Y, Jiang J P, Wang H T, et al. The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes. Bioresour Technol, 2015, 179: 483-489.
[23] Kobayashi N, Noel E A, Barnes A, et al. Rapid detection and quantification of triacylglycerol by HPLC-ELSD in Chlamydomonas reinhardtii and Chlorella Strains. Lipids, 2013, 48(10): 1035-1049.
[24] Li X B, Moellering E R, Liu B S, et al. A galactoglycerolipid lipase is required for triacylglycerol accumulation and survival following nitrogen deprivation in Chlamydomonas reinhardtii. Plant Cell, 2012, 24(11): 4670-4686.
[25] Simionato D, Block M A, La Rocca N, et al. The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus. Eukaryot Cell, 2013, 12(5): 665-676. |
