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| A Novel Aptasensor for Electrochemistry Detection of β-agonists |
| YANG Min1,3, CHEN Dan2,3, YAO Dong-sheng2,3, XIE Chun-fang1,3, LIU Da-ling1,3 |
1. College of Life Science and Technology, Jinan University, Guangzhou 510632, China;
2. National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China;
3. Institute of Microbial Technology, Jinan University, Guangzhou 510632, China |
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Abstract β-agonists are phenylethanolamines with different substituent groups on the aromatic ring and the terminal amino group which have the effect of nutrition redistribution and could accumulate in body tissues causing acute or chronic poisoning when consumed. Therefore, establishing a method for rapid screening of β-agonists is very important to food safety control. The aptamer named AP-Ago is screened by Isothermal Titration Calorimetric method. AP-Ago is a single-strand DNA and 22 base-pairs. The dissociation constant (Kd) to PHL is 3.34×10-5 mol/L. A labeled free electrochemical aptasensor is developed with AP-Ago, which is sensitive to phenylethanolamine (PHL), clenbuterol (CLB), ractopamine (RAC), salbutamol (SAL) and procaterol (PRO). The detection limits are 0.04ng/ml (RAC), 0.35pg/ml (CLB), 1.0pg/ml (PHL), 0.53pg/ml (SAL) and 1.73pg/ml (PRO), respectively. The detection time is 15min. This aptasensor also have a good reproductivity of the relative standard deviation (RSD) at 2.09%. The aptasensor can be developed as a rapid screening means with β-agonists (may be one or more) in sample.
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Received: 24 June 2015
Published: 24 November 2015
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