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| The Efficacy of PolyI:C and IL-15 as Adjuvant the Murine Zona Pellucid 3 DNA Vaccine Intranasally Immunized |
| WANG Dan-yang1, YANG Yu1, YANG Xiu-mei1, ZHANG Fu-chun1, WU Dao-cheng2, ZHANG Ai-lian1 |
1 College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
2 School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China |
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Abstract Aim:To observe the adjuvant efficacy of murine zona pellucid 3(mZP3) DNA vaccine through intranasal vaccination with PolyI:C and IL-15, and chitosan as delivery vector. Methods: Mice were delivered with chitosan-loaded mZP3 DNA vaccine or added PolyI:C and IL-15 by intranasal route, and to detect specific IgG antibody level in serum, sIgA in vaginal fluid and lung fluid by indirect ELISA, to detect lymphocyte proliferation by MTT, to do histology analysis of mice ovary for morphology of ovary. Results: PolyI:C and IL-15 can promote IgG antibody level of immunized mZP3 mice, promote lymphocyte proliferation and ovarian abnormalities in mice. Conclusion: The results suggest that antagonist of TLR PolyI:C and IL-15 have a synergistic effect and may be enhance the immunity effect of mZP3 DNA vaccine and reduce murine fertility rate to some extent.
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Received: 10 April 2015
Published: 25 September 2015
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