Optimization of Fermentation Medium for Creatininase Production by <em>Arthrobacter nicotiana</em>

doi:10.13523/j.cb.20150813

China Biotechnology

2015, Vol. 35

Issue (8): 90-95 DOI: 10.13523/j.cb.20150813

Optimization of Fermentation Medium for Creatininase Production by Arthrobacter nicotiana

LIANG Jiang-guang, JIA Jia-hong, WU Jun-wei, ZHU Jie

School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China

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Abstract

The optimal culture medium of creatininase production on the Arthrobacter nicotiana were carried out. Firstly, using creatine as inductor and metal ions experiment, it was found that creatine and metal ions have a great influence upon the creatininase production. Secondly, single factor experiment showed the optimization carbon and nitrogen sources was soluble starch and corn steep liquor,but compound nitrogen source of yeast extract and corn steep liquor was much better(creatininase production reached 108.5 U/g wet mycelium). At last, creatininase production reached 182.5 U/g wet mycelium under the optimization of fermentation medium, which was about 19.87 times as high as the initial fermentation enzyme activity.

Key words： Arthrobacter nicotiana Creatininase Optimization of production medium

Received: 23 March 2015 Published: 25 August 2015

ZTFLH:

Q93-335

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Cite this article:

LIANG Jiang-guang, JIA Jia-hong, WU Jun-wei, ZHU Jie . Optimization of Fermentation Medium for Creatininase Production by Arthrobacter nicotiana. China Biotechnology, 2015, 35(8): 90-95.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150813 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I8/90

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