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| Effects of proC and putP Deletion on Physiological Metabolism of L-arginine-producing Strain Corynebacterium crenatum |
| WAN Fang1, ZHANG Bin1, CHEN Min-liang2, CHEN Jin-cong2, CHEN Xue-lan1 |
1. College of Life Science, Jiangxi Normal University, Nanchang 330022, China;
2. College of Life Science, Nanchang University, Nanchang 330047, China |
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Abstract The recombinant strains of C. crenatum MT-M4 ΔproC and C. crenatum MT-M4 ΔputP were separately constructed based on genome-scale metabolic network model (GSMN) of Corynebacterium glutamicum. As results shown, L-arginine production of C. crenatum MT-M4 ΔproC was significantly increased by approximately 15.90% higher than that of the original strain, reached to 9.94g/L with glucose transformation rate increased by 26.02%. However, the growth of C. crenatum MT-M4 ΔproC was obviously inhibited. When 24 mmol/L of proline was added, the arginine production reached to 12.22g/L and its growth also got well. The L-arginine production of C. crenatum MT-M4 ΔputP was significantly increased by 42.70% and reached to 12.23g/L with glucose transformation rate increased by 49.31%. The results showed that putP deletion compared with proC deletion was more conducive to L-arginine biosynthesis. The disruption of putP had no influence on the physiological metabolism of the bacteria strain and the mutant strain did not need proline added in medium.
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Received: 19 March 2015
Published: 25 August 2015
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