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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (7): 62-67    DOI: 10.13523/j.cb.20150709
    
A Microcarrier Cell Culture Process for propagating Porcine Parvovirus in PK-15 Cells
LI Zhi-li, YI Xiao-ping, CHU Ju, ZHUANG Ying-ping, ZHANG Si-liang
East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Shanghai 200237, China
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Abstract  

In recent years, roller bottle was still used to produce porcine parvovirus. However, it was inefficient, labor-intensive and covered large areas. Now based on the optimization of time of infection (TOI), multiplicity of infection (MOI) and harvest time, porcine parvovirus static production process with PK-15 cells was well developed, that the maximum virus titre could reach 107.5 TCID50/ml. More specifically, by optimizing TOI, the process was successfully transferred from static culture to microcarrier suspension culture, and 5L bioreactor verification experiment was conducted, which enable the maximum virus titer reached 107.2 TCID50/ml. It was the first time to verify that the yield coefficients of lactate to glucose had a positive correlation with virus titre, that it could reach the peak value as soon as the porcine parvovirus at the maximum virus titre. It can be used as an important parameter for virus harvest.



Key wordsPK-15 cells      Porcine parvovirus      Microcarrier      Stirred bioreactor     
Received: 02 April 2015      Published: 25 July 2015
ZTFLH:  Q813  
Cite this article:

LI Zhi-li, YI Xiao-ping, CHU Ju, ZHUANG Ying-ping, ZHANG Si-liang. A Microcarrier Cell Culture Process for propagating Porcine Parvovirus in PK-15 Cells. China Biotechnology, 2015, 35(7): 62-67.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150709     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I7/62


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