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| In vitro functionality and in-vivo Safety Assessment of Bone Repair Scaffold Using 3D Printing Technology |
| ZHANG Deng-yang, ZHANG Ying, ZHANG Li-jun, WANG Yan |
| School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, China |
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Abstract Scaffold was produced by using 3D printer, its functionality of supporting the differentiation of hMSC and in vivo safety was tested. Method: 3D PLGA/HA composite scaffold was printed with 3D printer, the mechanical strength of the 3D scaffold was tested according to the GB/T1040 and GB/T 9341.Its ability of supporting hMSC multiplication and differentiation was validated in vitro, finally the material was assessed of its in vivo safety based on the GB/T 16886.Result: Porous PLGA/HA 3D scaffold was successful made, whose tensile-strength and bending strength is 38MPa and 42MPa, which is about 5.35 and 5.25 times more than that of human cartilage. The 3D material supports the Chondrogenic differentiation of hMSC. Biological safety experiment results proved that the 3D material accord with the standard of medical devices.
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Received: 11 March 2015
Published: 25 July 2015
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