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14-3-3γ Regulates the Physiological Function of Dairy Cow Mammary Gland Epithelial Cells Through mTOR Signaling Pathway
LIU Jing, LUO Chao-chao, HUANG Jian-guo, WU Di, GAO Xue-jun, LIU Yu-fen
China Biotechnology ›› 2015, Vol. 35 ›› Issue (6) : 32-39.
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14-3-3γ Regulates the Physiological Function of Dairy Cow Mammary Gland Epithelial Cells Through mTOR Signaling Pathway
As a linker of proteins, 14-3-3γ plays critical roles in cell signal transductions, cell growth,differentiation and protein synthesis. Based on cultured dairy cow mammary gland epithelial cells in vitro, the impact of 14-3-3γ on the physiological function of dairy cow mammary gland epithelial cells were ivestigated. Expressing plasmids of 14-3-3γ gene were constructed, then stably and transiently transfected into dairy cow mammary gland epithelial cells. The effect of over-expression on dairy cow mammary gland epithelial cells was analyzed by CASY. Triglyceride was detected by triglyceride GPO-POD assay and beta casein was detected by high performance liquid chromatography. Expression of mTOR and p-mTOR was detected by Western blot. Experimental results show that the viability of dairy cow mammary gland epithelial cells, the expression of triglyceride and β-casein, the expression of mTOR and p-mTOR was identified up-expressed(P<0.01). All above results indicated that 14-3-3γ regulates the physiological function of dairy cow mammary gland epithelial cells through mTOR signaling pathway. The result enriches the lactation signaling pathway, which provides theoretical basis for improving the quality of milk.
14-3-3γ / Dairy cow / Mammary gland epithelial cells / mTOR / Signaling pathway {{custom_keyword}} /
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