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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (4): 66-73    DOI: 10.13523/j.cb.20150410
    
Multiplex Taqman Real-time PCR for Detection of Malignant Tumor Metastasis Rate in Cancer Metastasis Model in Nude Mice
ZHANG Yu, BAI Dou, ZHU Nai-shuo
Lab of Molecular Immunology, State Key Lab of Genetic Engineering, Institute of Biomedical Science, School of Life Science, Fudan University, Shanghai 200438, China
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Abstract  

Objective: To make the efficient detection of malignant tumor metastasis rate in cancer metastasis in animal models by use of the multiplex real-time fluorescence quantitative PCR. Methods: The species-specific primers and probes for detection of beta-2-macroglobulin(β2m) gene of human beings and mouse in multiplex real-time fluorescence quantitative PCR assay was designed. Total DNA as templates were extracted respectively from human ACC cells and mouse Sp2/0 cells. The specificity and sensitivity of these primer pairs were separately confirmed using simplex real-time PCR analysis. The specificity, sensitivity and accuracy of the multiplex real-time fluorescence quantitative PCR method were evaluated and compared with the traditional method by weighing tissues of tumor metastasis to calculate the rate of tumor metastasis. Results: The limit of detection (LOD) of the assay was 0.006ng/μl DNA for each target species (1:104 dilution). Conclusions: This system proved its accuracy, precision and applicability for the detection of malignant tumor metastasis rate in animal models.

Key words： Multiplex real-time fluorescence quantitative PCR      Tumor metastasis rate      ACCM     
Received: 19 October 2014      Published: 25 April 2015
ZTFLH:  Q819  
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Cite this article:

ZHANG Yu, BAI Dou, ZHU Nai-shuo. Multiplex Taqman Real-time PCR for Detection of Malignant Tumor Metastasis Rate in Cancer Metastasis Model in Nude Mice. China Biotechnology, 2015, 35(4): 66-73.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150410     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I4/66


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