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| Preparation of Photosensitizer-loaded Cubic Liquid Crystalline and Its Photodynamic Therapy Effects |
| FU Huai-xiu1,2, YU Xiang1, KANG Hong-xiang2, LIANG Jie2, CHEN Peng2, SHEN Ben-jian2, JIN Yi-guang2, XION Li3, MAO Jian-ping1,2 |
1. Graduate School of Anhui Medical University, Hefei 230032, China;
2. Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China;
3. Department of General Surgery, Second Xiang Hospital, Central South University, Changsha 410083, China |
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Abstract Objectives: To prepare photosensitizers-loaded Liquid Crystalline Nanoparticles, and study its cytotoxicity and photodynamic therapy (PDT) effects on tumor cells. Methods: Photosan Cubosomes were prepared with Glyceryl Monooleate (GMO), Poloxamer 407 (P407) and Photosan. Then the cubosomes were observed on the dynamic light scattering and scanning microscope. The photodymanic therapy effects and cytotoxicity of Photosan Cubosomes to L02 cells and HepG2 cells were studied by MTS assay. Results: Photosan Cubosomes were prepared successfully. The results showed that the blank cubosomes, Photosan and Photosan Cubosomes did not display any cellular toxicity against L-02 cells and HepG2 cells without laser irradiating; however, Liquid Crystalline Photosan mediated PDT by 5J/cm2 laser produced obvious inhibitory effects on HepG2 cells [inhibited (77.9±2.06)%] while showed slight inhibitory effects on L-02 cells [inhibited (32.9±1.19)%]. Moreover, Liquid Crystalline PhotosanPDT showed less damage on L-02 cells but stronger inhibitory effects on HepG2 than Photosan. Conclusions: New photosensitizer loaded Photosan Cubic Liquid Crystalline had higher security and stronger PDT effects on cancer cells and showed better characteristics than Photosan. It provided a novel method for cancer treatment by PDT.
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Received: 30 December 2014
Published: 25 March 2015
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Cite this article:
FU Huai-xiu, YU Xiang, KANG Hong-xiang, LIANG Jie, CHEN Peng, SHEN Ben-jian, JIN Yi-guang, XION Li, MAO Jian-ping. Preparation of Photosensitizer-loaded Cubic Liquid Crystalline and Its Photodynamic Therapy Effects. China Biotechnology, 2015, 35(3): 35-41.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150305 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I3/35
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