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| The Breeding of High-yielding Actinoplanes of Rapamycin |
| SHI Man-man1, QIAO Chang-sheng1,2, ZHU Ming1, LI Xue2, LIU Shan-shan1 |
1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Peiyang Biotrans Co., Ltd, Tianjin 300457, China |
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Abstract The Actinoplanes of BCLP-016 was selected as the original strain, and a mutagenesis of atmospheric and room temperature plasma (ARTP) was used to treat its spores, the spore suspension treated with three different time was mixed. After dilution coated, picking some single colonies according to the colony morphology of the strain for screening, after fermentation rescreening, a high-yielding strain of rapamycinARTP-039 was screened out, its rapamycin production can reach 369.39mg/L, compared with the yield of the original strain BCLP-016 256.86 mg/L, increased by 43.81%.Choosing the high-yielding strain ARTP-039 as the starting strain, the traditional UV mutagenesis was conducted, three time that corresponding with fatality rate was selected to treat its spore suspension, based on the theory of the ribosome engineering, picking out five kinds of resistant material of streptomycin, gentamicin, rifampicin, chloramphenicol and erythromycin to conduct the resistance screening. After fermentation rescreening, a high-yielding strain of St8+Gen6+Rif9+Chl3+Er4-015 was screened out, it has five kinds of resistance. The strain fermentation test showed that after 7 days of fermentation, the rapamycin output can reach 589.79mg/L, compared with the original strain BCLP-016 yield increased by 129.61%,and its genetic stability is good.
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Received: 22 October 2014
Published: 25 February 2015
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