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| The Impacts of Feeder Cells on Culture in vitro of Sheep Embryonic Stem Cells |
| ZHAO Rui-yuan, LIU Chun-xia, LI Hui-peng, WANG Shen-yuan, ZHOU Huan-min |
| College of Life Science, Inner Mongolia Agricultural University; Key Laboratory of Biological Manufacturing Inner Mongolia Auto Region, Hohhot 010018, China |
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Abstract Embryonic stem cell (ESC) research progresses show slowly on many species, especially on livestock. Although sheep ESC research has long been reported, but not yet established stabilize passaged cell lines. On the basis of the establishing system in vitro fertilization and development of sheep, the experiment explored the impact of feeder layers (Feeder) cells on sheep ESC, including processing time of feeder on certain concentration of mitomycin, cell seeding density, cell species, cell passages, and feeder cells freshly prepared and frozen. The aim of the comparative study is to optimize the appropriate feeder cells for the establishment of sheep ESC culture system in vitro. The results showed that the first to the fifth passage of SEF and the first to the third passage MEF and the 1:1 mixture of both cells which were treated by 10 μg/ml mitomycin for 2~2.2 hours could better support the growth of sheep ESC.
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Received: 04 November 2014
Published: 25 February 2015
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