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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (2): 8-17    DOI: 10.13523/j.cb.20150202
    
Killing Effects of Thioridazine on Liver Cancer Stem Cell
MENG Shu-lin, MA Bu-yun, ZHANG Xin-min, GE Yun, ZHANG Rong, HUANG Pan-pan, WANG Yi-gang
School of Life Sciences, Xinyuan Institute of Medicine and Biotechnology, Zhejiang Sci-Tech University, Hangzhou 310018, China
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Abstract  

Thioridazine (THO) was originally used to treat psychotic patients. Recently, THO was shown to inhibit the growth of many cancer cell lines.But its killing effect on liver cancer stem cells have not yet been reported. Cancer stem cells play an important role in tumor metastasis, recurrence and drug resistance. The suspension culture was used to enrich cancer stem cells in vitro to test killing effect of THO on liver cancer stem cell. Liver cancer cells that cultured in suspension medium are detected similarly to liver cancer stem cells in many aspects, including embryonic transcription factors dependence, drug resistance and cell cycle arrest;which show that it significantly upregulated the expression of phosphorylated STAT3, NANOG and XIAP,and decreased expression of albumin proteins.The cytotoxicity of THO on liver cancer stem cells were evaluated by MTT, Western blotting and flow cytometry assay. The results indicate that THO can significantly inhibit the proliferation of liver cancer stem cells and induce caspase-dependent apoptosis;but weakly to liver cancer cells. However, the combination of THO and doxorubicin hydrochloride(DOX) could more significantly inhibit the proliferation of liver cancer stem cells and caner cells similarly. The results show that THO has a specific strong killing effect on liver cancer stem cells, and may bring new hope for future clinical treatment of liver cance.



Key wordsTHO      Liver cancer stem cells      Phosphorylated STAT3      Caspase      Apoptosis     
Received: 18 November 2014      Published: 25 February 2015
ZTFLH:  Q819  
Cite this article:

MENG Shu-lin, MA Bu-yun, ZHANG Xin-min, GE Yun, ZHANG Rong, HUANG Pan-pan, WANG Yi-gang. Killing Effects of Thioridazine on Liver Cancer Stem Cell. China Biotechnology, 2015, 35(2): 8-17.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150202     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I2/8


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