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| The Expression of OPCML in Gastric Cancer and Role of OPCML in Biological Function of Gastric Cancer Cell |
| LI Ming1, WANG Yong-fei1, JIAO Jin-xia1, YANG Yang1, ZHANG Ning3, XING Xiang-bin2, MA San-mei1 |
1. Department of Biotechnology, Jinan University, Guangzhou 510632, China;
2. Department of Gastroenterology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;
3. First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, China |
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Abstract Purpose: To investigate the association of OPCML with clinicopathological factors and prognosis of gastric cancer, and analyze the effect of OPCML gene on biological behavior of AGS. Methods: (1) The expression of OPCML protein was detected by immunohistochemistry in 118 cases of gastric carcinoma, and evaluate the correlation of OPCML with clinicopathological characteristics and prognosis of gastric cancer. (2) The OPCML expression in normal tissues and 7 kinds of gastric cells was assessed by RT-PCR. (3) The recombinant plasmid pcDNA3.1-OPCML was constructed and transfected into AGS cells. Using CCK8, colony formation assay to analysis the impact on the biologic- al behavior of the AGS after overexpression of OPCML. Results: (1) Immunohistochemistry showed the low expression of OPCML in gastric cancer accounted for 68.6%, and OPCML expression had a significant inverse correlation with depth of tumor invasion and tumor differentiation degree (P<0.05); OPCML expression, depth of tumor invasion, lymph node metastasis and distant metastasis were important factors affecting the prognosis of patients survival (P<0.05). (2) OPCML mRNA expression in gastric cancer cells was significantly lower than in normal gastric mucosa. (3) RT-PCR showed that the OPCML expression was aberrantly increased in cells transfected with pcDNA3.1-OPCML. CCK8, colony formation assay showed that OPCML significantly inhibited the growth, proliferation, colony of AGS cells. Conclusions: OPCML play a role as tumor suppressor in gastric cancer, and it may be a new prognostic indicator of gastric cancer.
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Received: 13 November 2014
Published: 25 February 2015
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